Statistical screenings of medium components for the production of chitinase by the marine isolate Pantoea dispersa

被引：43

作者：

Gohel, V

Chaudhary, T

Vyas, P

Chhatpar, HS ^{[1
]}

机构：

[1] Maharaja Sayajirao Univ Baroda, Fac Sci, Dept Microbiol, Baroda 390002, Gujarat, India

[2] Maharaja Sayajirao Univ Baroda, Fac Sci, Biotechnol Ctr, Baroda 390002, Gujarat, India

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2006年 / 28卷 / 01期

关键词：

biomass; chitinase; Pantoea dispersa; pH; Plackett-Burman;

D O I：

10.1016/j.bej.2005.09.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Effect of 19 different medium components on chitinase production by marine isolate Pantoea dispersa was studied by Plackett-Burman design. Each component was screened on the basis of 'p' (probability) value, which was at 95% or above of confidence level. In the initial chitinase production medium containing five medium components chitinase production of 108 units/ml was obtained. In the Plackett-Burman method, significant factors influencing the chitinase production were screened. 4.21-fold increase in chitinawse production was observed in 22nd medium of Plackett-Burman experimental design. Increase in production of endochitinase (3.95-fold) and chitobiase (2.31-fold) was observed. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：50 / 56

页数：7

共 40 条

[1]

L-asparaginase production by Pseudomonas aeruginosa in solid-state culture:: evaluation and optimization of culture conditions using factorial designs [J].