Extracellular vesicles in chronic lymphocytic leukemia

被引:13
作者
Haderk, Franziska [1 ]
Hanna, Bola [1 ]
Richter, Karsten [2 ]
Schnoelzer, Martina [3 ]
Zenz, Thorsten [4 ,5 ,6 ]
Stilgenbauer, Stephan [7 ]
Lichter, Peter [1 ]
Seiffert, Martina [1 ]
机构
[1] German Canc Res Ctr, Dept Mol Genet, D-69120 Heidelberg, Germany
[2] German Canc Res Ctr, Unit Electron Microscopy, Imaging & Cytometry Core Facil, D-69120 Heidelberg, Germany
[3] German Canc Res Ctr, Res Grp Funct Proteome Anal, D-69120 Heidelberg, Germany
[4] Natl Ctr Tumor Dis NCT, Dept Translat Oncol, Heidelberg, Germany
[5] German Canc Res Ctr, D-69120 Heidelberg, Germany
[6] Heidelberg Univ, Dept Med 5, Heidelberg, Germany
[7] Univ Ulm, Dept Internal Med 3, D-89069 Ulm, Germany
关键词
extracellular vesicles; microvesicles; exosomes; chronic lymphocytic leukemia; proteome; microenvironment; CELLS IN-VITRO; B-CELLS; MICROVESICLES; PROGRESSION; COMMUNICATION; FIBRONECTIN; APOPTOSIS; MEDIATORS; SURVIVAL; EXOSOMES;
D O I
10.3109/10428194.2013.796052
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Extracellular vesicles (EVs) are membrane-enclosed nanoparticles 30 to 1000 nm in size and represent a novel mechanism of cell communication. By transferring RNA and protein from their cell of origin, they can reprogram target cells and thus are involved in changes within the cellular microenvironment - a key player in CLL pathogenesis. In the current study, we were able to isolate EVs of 20 to 300 nm from blood plasma of CLL patients as well as from supernatant of primary CLL cells in culture. Further, proteomic profiling by Coomassie staining of SDS-PAGE gels and by mass spectrometry revealed an EV-specific protein profile. These findings suggest that EVs represent an important mean of CLL cells to interact with other cells, which might contribute to the establishment of a pro-survival microenvironment for CLL cells.
引用
收藏
页码:1826 / 1830
页数:5
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