UV irradiation as a tool for obtaining asymmetric somatic hybrids between Nicotiana plumbaginifolia and Lycopersicon esculentum

被引：36

作者：

Vlahova, M ^{[1
]}

Hinnisdaels, S ^{[1
]}

Frulleux, F ^{[1
]}

Claeys, M ^{[1
]}

Atanassov, A ^{[1
]}

Jacobs, M ^{[1
]}

机构：

[1] FREE UNIV BRUSSELS,B-1640 RHODE ST GENESE,BELGIUM

来源：

THEORETICAL AND APPLIED GENETICS | 1997年 / 94卷 / 02期

关键词：

asymmetric cell hybridization; UV irradiation; chromosome elimination; Nicotiana; Lycopersicon;

D O I：

10.1007/s001220050398

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

UV-irradiated kanamycin-resistant Lycopersicon esculentum leaf protoplasts were fused with wild-type Nicotiana plumbaginifolia leaf protoplasts. Hybrid calli were recovered after selection in kanamycin-containing medium and subsequently regenerated. Cytological analysis of these regenerants showed that several (2-4) tomato chromosomes, or chromosome fragments, were present in addition to a polyploid Nicotiana genome complement. All lines tested had neomycin phosphotransferase (NPTII) activity and the presence of the kanamycin gene was shown by Southern blotting. In two cases a different hybridization profile for the kanamycin gene, compared to the tomato donor partner, was observed, suggesting the occurence of intergenomic recombination events. The hybrid nature of the regenerants was further confirmed by Southern-blotting experiments using either a ribosomal DNA sequence or a tomato-specific repeat as probes. The hybrids were partially fertile and some progeny could be obtained. Our results demonstrate that UV irradiation is a valuable alternative for asymmetric cell-hybridization experiments.

引用

页码：184 / 191

页数：8

共 43 条

[1]

ATANASSOV A, 1991, PHYSIOL PLANTARUM, pA23

[2] ASYMMETRIC HYBRIDIZATION IN NICOTIANA BY FUSION OF IRRADIATED PROTOPLASTS [J].