Functional and structural characterization of chimeras of a bacterial genotoxin and human type I DNAse

被引：15

作者：

DiRienzo, Joseph M. ^{[1
]}

Cao, Linsen ^{[1
]}

Volgina, Alla ^{[1
]}

Bandelac, Georges ^{[2
]}

Korostoff, Jonathan ^{[2
]}

机构：

[1] Univ Penn, Sch Dent Med, Dept Microbiol, Philadelphia, PA 19104 USA

[2] Univ Penn, Sch Dent Med, Dept Periodont, Philadelphia, PA 19104 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2009年 / 291卷 / 02期

基金：

美国国家卫生研究院;

关键词：

Aggregatibacter actinomycetemcomitans; chimera; cytolethal distending toxin; epithelial cells; pathogen; type I DNAse; CYTOLETHAL DISTENDING TOXIN; CELL-CYCLE ARREST; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; DEOXYRIBONUCLEASE-I; CYSTIC-FIBROSIS; SUBUNIT; CDTB; INTERNALIZATION; SPECIFICITY; INVOLVEMENT;

D O I：

10.1111/j.1574-6968.2008.01457.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

Chimeras composed of the cdtB gene of a novel bacterial genotoxin and the human type I DNAse I gene were constructed and their products characterized relative to the biochemical and enzymatic properties of the native proteins. The product of a cdtB/DNAse I chimera formed a heterotrimer with the CdtA and CdtC subunits of the genotoxin, and targeted mutations increased the specific activity of the hybrid protein. Expression of active chimeric gene products established that the CdtB protein is an atypical divalent cation-dependent endonuclease and demonstrated the potential for genetically engineering a new class of therapeutic agent for inhibiting the proliferation of cancer cells.

引用

页码：222 / 231

页数：10

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