Pseudomonas aeruginosa quorum-sensing signal molecules interfere with dendritic cell-induced T-cell proliferation

被引:83
作者
Skindersoe, Mette E. [1 ]
Zeuthen, Louise H. [2 ]
Brix, Susanne [2 ]
Fink, Lisbeth N. [2 ]
Lazenby, James [3 ]
Whittall, Christine [3 ]
Williams, Paul [4 ]
Diggle, Stephen P. [4 ]
Froekiaer, Hanne [2 ]
Cooley, Margaret [3 ]
Givskov, Michael [1 ]
机构
[1] Tech Univ Denmark, Ctr Biomed Microbiol, DK-2800 Lyngby, Denmark
[2] Tech Univ Denmark, Ctr Biol Sequence Anal, Nutr Immunol Grp, Dept Syst Biol, DK-2800 Lyngby, Denmark
[3] Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW, Australia
[4] Univ Nottingham, Inst Infect Immun & Inflammat, Ctr Biomol Sci, Nottingham NG7 2RD, England
来源
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 2009年 / 55卷 / 03期
基金
英国生物技术与生命科学研究理事会;
关键词
dendritic cell; immunomodulation; OdDHL; PQS; Pseudomonas aeruginosa; quorum sensing; NF-KAPPA-B; CYSTIC-FIBROSIS PATIENTS; PPAR-GAMMA AGONISTS; N-(3-OXODODECANOYL)-L-HOMOSERINE LACTONE; HOMOSERINE LACTONE; EPITHELIAL-CELLS; IN-VIVO; ACYLHOMOSERINE LACTONES; ESCHERICHIA-COLI; MAMMALIAN-CELLS;
D O I
10.1111/j.1574-695X.2008.00533.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Pseudomonas aeruginosa releases a wide array of toxins and tissue-degrading enzymes. Production of these malicious virulence factors is controlled by interbacterial communication in a process known as quorum sensing. An increasing body of evidence reveals that the bacterial signal molecule N-(3-oxododecanoyl)-l-homoserine lactone (OdDHL) exhibits both quorum-sensing signalling and immune-modulating properties. Recently, yet another quorum-sensing signal molecule, the Pseudomonas quinolone signal (PQS), has been shown to affect cytokine release by mitogen-stimulated human T cells. In the present article we demonstrate that both OdDHL and PQS decrease the production of interleukin-12 (IL-12) by Escherichia coli lipopolysaccharide-stimulated bone marrow-derived dendritic cells (BM-DCs) without altering their IL-10 release. Moreover, BM-DCs exposed to PQS and OdDHL during antigen stimulation exhibit a decreased ability to induce T-cell proliferation in vitro. Collectively, this suggests that OdDHL and PQS change the maturation pattern of stimulated DCs away from a proinflammatory T-helper type I directing response, thereby decreasing the antibacterial activity of the adaptive immune defence. OdDHL and PQS thus seem to possess dual activities in the infection process: as inducers of virulence factors as well as immune-modulators facilitating the infective properties of this pathogen.
引用
收藏
页码:335 / 345
页数:11
相关论文
共 64 条
[1]   PPAR-γ agonists inhibit toll-like receptor-mediated activation of dendritic cells via the MAP kinase and NF-κB pathways [J].
Appel, S ;
Mirakaj, V ;
Bringmann, A ;
Weck, MM ;
Grünebach, F ;
Brossart, P .
BLOOD, 2005, 106 (12) :3888-3894
[2]   IMMUNOHISTOPATHOLOGIC LOCALIZATION OF PSEUDOMONAS-AERUGINOSA IN LUNGS FROM PATIENTS WITH CYSTIC-FIBROSIS - IMPLICATIONS FOR THE PATHOGENESIS OF PROGRESSIVE LUNG DETERIORATION [J].
BALTIMORE, RS ;
CHRISTIE, CDC ;
SMITH, GJW .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1989, 140 (06) :1650-1661
[3]   EXPRESSION OF T-CELL RECEPTOR ALPHA-CHAIN GENES IN TRANSGENIC MICE [J].
BERG, LJ ;
FAZEKAS DE ST GROTH, B ;
IVARS, F ;
GOODNOW, CC ;
GILFILLAN, S ;
GARCHON, HJ ;
DAVIS, MM .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (12) :5459-5469
[4]   THE BIOFLAVONOID QUERCETIN INHIBITS NEUTROPHIL DEGRANULATION, SUPEROXIDE PRODUCTION, AND THE PHOSPHORYLATION OF SPECIFIC NEUTROPHIL PROTEINS [J].
BLACKBURN, WD ;
HECK, LW ;
WALLACE, RW .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 144 (03) :1229-1236
[5]   Significant immunomodulatory effects of Pseudomonas aeruginosa quorum-sensing signal molecules: possible link in human sepsis [J].
Boontham, Pisake ;
Robins, Adrian ;
Chandran, Palanichamy ;
Pritchard, David ;
Camara, Miguel ;
Williams, Paul ;
Chuthapisith, Suebwong ;
McKechnie, Alasdair ;
Rowlands, Brian J. ;
Eremin, Oleg .
CLINICAL SCIENCE, 2008, 115 (11-12) :343-351
[6]   A novel and sensitive method for the quantification of N-3-oxoacyl homoserine lactones using gas chromatography-mass spectrometry:: application ito a model bacterial biofilm [J].
Charlton, TS ;
de Nys, R ;
Netting, A ;
Kumar, N ;
Hentzer, M ;
Givskov, M ;
Kjelleberg, S .
ENVIRONMENTAL MICROBIOLOGY, 2000, 2 (05) :530-541
[7]   Synthetic analogues of the bacterial signal (quorum sensing) molecule N-(3-oxododecanoyl)-L-homoserine lactone as immune modulators [J].
Chhabra, SR ;
Harty, C ;
Hooi, DSW ;
Daykin, M ;
Williams, P ;
Telford, G ;
Pritchard, DI ;
Bycroft, BW .
JOURNAL OF MEDICINAL CHEMISTRY, 2003, 46 (01) :97-104
[8]   Inactivation of a Pseudomonas aeruginosa quorum-sensing signal by human airway epithelia [J].
Chun, CK ;
Ozer, EA ;
Welsh, MJ ;
Zabner, J ;
Greenberg, EP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (10) :3587-3590
[9]   DNA REPLICATION AND DIVISION CYCLE IN ESCHERICHIA COLI [J].
CLARK, DJ ;
MAALOE, O .
JOURNAL OF MOLECULAR BIOLOGY, 1967, 23 (01) :99-&
[10]   A bacterial cell to cell signal in the lungs of cystic fibrosis patients [J].
Collier, DN ;
Anderson, L ;
McKnight, SL ;
Noah, TL ;
Knowles, M ;
Boucher, R ;
Schwab, U ;
Gilligan, P ;
Pesci, EC .
FEMS MICROBIOLOGY LETTERS, 2002, 215 (01) :41-46