The affinity of binding of digoxin to ovine anti-digoxin Fab (DIGIBIND(TM)) preparations

被引：11

作者：

Allen, G ^{[1
]}

机构：

[1] WELLCOME RES LABS,DIV BIOL,BECKENHAM BR3 3BS,KENT,ENGLAND

来源：

BIOLOGICALS | 1996年 / 24卷 / 01期

关键词：

MONOCLONAL-ANTIBODIES; DIGITALIS INTOXICATION; ANTIDIGOXIN ANTIBODIES; SPECIFICITY; FRAGMENTS; GLYCOSIDE; KINETICS;

D O I：

10.1006/biol.1996.0002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fundamental study was performed to assess the binding characteristics of an ovine antidigoxin-fab preparation, DIGIBIND(TM), for digoxin, using equilibrium dialysis. The average mass of the antibody fragment was determined by matrix-assisted laser-desorption mass spectrometry to be 46 200 Da. The main conclusions are that the binding affinity and capacity are similar for several batches, produced over a period of several years. The average median affinity for digoxin of five batches determined by Sips analysis was 3.4 x 10(11) M(-1) (range 1.95-6.13 x 10(11) M(-1)) (corresponding to dissociation constants, Kd, in the range 1.63-5.13 pM), and the index of heterogeneity was 0.78 +/- 0.03. (C) 1996 The International Association of Biological Standardization

引用

页码：19 / 24

页数：6

共 22 条

[1]

BALTHASAR J, 1994, J PHARMACOL EXP THER, V268, P734

[2]

BERZOFSKY JA, 1989, FUNDAMENTAL IMMUNOLO, P315

[3] DIGOXIN AND DIGOXIN DERIVATIVE INDUCED ARRHYTHMIAS - INVITRO BINDING AND INVIVO ABOLITION OF ARRHYTHMIAS BY DIGOXIN IMMUNE FAB (DIGIBAND) [J].

CAMBRIDGE, D ;

MORGAN, CR ;

ALLEN, G .

CARDIOVASCULAR RESEARCH, 1992, 26 (09) :906-911

[4] ISOLATION OF DIGOXIN-SPECIFIC ANTIBODY AND ITS USE IN REVERSING EFFECTS OF DIGOXIN [J].

CURD, J ;

SMITH, TW ;

JATON, JC ;

HABER, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1971, 68 (10) :2401-&

[5]

DANIELSSON L, 1991, IMMUNOLOGY, V74, P50

[6]

EHRLICH PH, 1988, CLIN CHEM, V34, P1681

[7]

FUJII Y, 1994, CLIN CHEM, V40, P172

[8] DIGOXIN IMMUNE FAB THERAPY IN THE MANAGEMENT OF DIGITALIS INTOXICATION - SAFETY AND EFFICACY RESULTS OF AN OBSERVATIONAL SURVEILLANCE STUDY [J].

HICKEY, AR ;

WENGER, TL ;

CARPENTER, VP ;

TILSON, HH ;

HLATKY, MA ;

FURBERG, CD ;

KIRKPATRICK, CH ;

STRAUSS, HC ;

SMITH, TW .

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 1991, 17 (03) :590-598

[9] MASS-SPECTROMETRY OF PEPTIDES AND PROTEINS BY MATRIX-ASSISTED ULTRAVIOLET-LASER DESORPTION IONIZATION [J].

HILLENKAMP, F ;

KARAS, M .

METHODS IN ENZYMOLOGY, 1990, 193 :280-295

[10]

HINDS JA, 1986, CLIN CHEM, V32, P16

← 1 2 3 →