Dynamic thin laminar flow method for making protein monolayers

This paper describes a new way to make protein monolayers rapidly. This process reduces the protein contact time at the air-water interface, which subsequently prevents structural alteration. High-speed protein monolayer preparations are obtained by confining proteins in a thin laminar flow liquid film at various speeds. A prototype made of a rotating glass cylinder compressing cytochrome P450(scc) validated this method. The cytochrome P450 monolayers were verified by optical microscopy, TEM, STM, and AFM. Possible protein 1D crystallites were observed. A rate of protein monolayer preparation up to 2 cm/s was easily obtained. This method is suitable for continuous protein monolayer production and line particle coating.