O-(fluoresceinylmethyl) hydroxylamine (OFMHA):: A reagent for the preparation of fluorescent O-(fluoresceinylmethyl)oxime (FMO)-steroid conjugates

被引：16

作者：

Adamczyk, M ^{[1
]}

Chen, YY ^{[1
]}

Grote, J ^{[1
]}

Mattingly, PG ^{[1
]}

机构：

[1] Abbott Labs, Dept Chem, Div Diagnost, Abbott Pk, IL 60064 USA

来源：

STEROIDS | 1999年 / 64卷 / 04期

关键词：

O-(fluoresceinylmethyl)hydroxylamine; fluorescein steroid conjugates; tracers;

D O I：

10.1016/S0039-128X(99)00004-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 5 and 6-isomers of O-(fluoresceinylmethyl)hydroxylamine reacted with a representative sample of oxo-steroids (6-oxoestradiol, estrone, norethindrone, cortisol, progesterone, and digitoxin-dialdehyde) to produce O-(fluoresceinylmethyl)oxime conjugates in a single step in 24-84% yield after preparative high performance liquid chromotography. (C) 1999 Elsevier Science Inc. All rights reserved.

引用

页码：283 / 290

页数：8

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