Fluorescence detection of single molecules near a solution/glass interface - an electrodynamic analysis

被引：21

作者：

Enderlein, J ^{[1
]}

机构：

[1] Univ Regensburg, Inst Analyt Chem Chemo & Biosensors, D-93040 Regensburg, Germany

来源：

CHEMICAL PHYSICS LETTERS | 1999年 / 308卷 / 3-4期

关键词：

D O I：

10.1016/S0009-2614(99)00595-3

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The apparent fluorescence intensity of single molecules in solution near a glass surface is studied theoretically. It is shown that a correct interpretation of experimental data has to take into account not only the spatial variation of the exciting light intensity but also the change of the molecule's fluorescence properties when it approaches the solution/glass surface. Numerical results are presented for the experimental conditions as used by Xu and Yeung [Science 281 (1998) 1650]. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：263 / 266

页数：4

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