Inhibition by light of CO2 evolution from dark respiration:: Comparison of two gas exchange methods

被引:47
作者
Peisker, M [1 ]
Apel, H [1 ]
机构
[1] IPK, D-06466 Gatersleben, Germany
关键词
A-c(i) curves; CO2 compensation concentration; day respiration; intracellular resistance; mesophyll resistance; night respiration; photocompensation point;
D O I
10.1023/A:1014799118368
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Two approaches to determine the fraction (mu) of mitochondrial respiration sustained during illumination by measuring CO2 gas exchange are compared. In single leaves, the respiration rate in the light (`day respiration' rate R d) is determined as the ordinate of the intersection point of A-c i curves at various photon flux densities and compared with the CO2 evolution rate in darkness (`night respiration' rate R n). Alternatively, using leaves with varying values of CO2 compensation concentration (Gamma), intracellular resistance (r i) and R n, an average number for mu can be derived from the linear regression between Gamma and the product r i.R n. Both methods also result in a number c* for that intercellular CO2 concentration at which net CO2 uptake rate is equal to -R d. c* is an approximate value of the photocompensation point Gamma* (Gamma in the absence of mitochondrial respiration), which is related to the CO2/O-2 specificity factor of Rubisco S c/o. The presuppositions and limitations for application of both approaches are discussed. In leaves of Nicotiana tabacum, at 22 degreesC, single leaf measurements resulted in mean values of mu = 0.71 and c(*) = 34 mumol mol(-1). At the photosynthetically active photon flux density of 960 mumol quanta m(-2) s(-1), nearly the same numbers were derived from the linear relationship between Gamma and r i.R n. c* and R d determined by single leaf measurements varied between 31 and 41 mumol mol(-1) and between 0.37 and 1.22 mumol m(-2) s(-1), respectively. A highly significant negative correlation between c* and R-d was found. From the regression equation we obtained estimates for Gamma* (39 mumol mol(-1)), S c/o (96.5 mol mol(-1)) and the mesophyll CO2 transfer resistance (7.0 mol(-1) m(2) s).
引用
收藏
页码:291 / 298
页数:8
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