Analysis of thiopurines using aqueous normal phase chromatography

被引：4

作者：

Pesek, Joseph J. ^{[1
]}

Matyska, Maria T. ^{[1
]}

Young, Joshua E. ^{[2
]}

机构：

[1] San Jose State Univ, Dept Chem, San Jose, CA 95112 USA

[2] MicroSolv Technol Corp, Eatontown, NJ 01776 USA

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2014年 / 95卷

关键词：

Silica hydride stationary phase; Hydrophilic analytes; Pharmaceutical analysis; Metabolites; PERFORMANCE LIQUID-CHROMATOGRAPHY; METHYL 6-MERCAPTOPURINE NUCLEOTIDES; INFLAMMATORY-BOWEL-DISEASE; RED-BLOOD-CELLS; HYDROPHILIC METABOLITES; 6-THIOGUANINE; AZATHIOPRINE; ASSAY; QUANTIFICATION; PEPTIDES;

D O I：

10.1016/j.jpba.2014.02.019

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The chromatography of several thiopurines is investigated using aqueous normal phase (ANP) conditions in conjunction with a silica hydride-based column. Both isocratic and gradient elution modes are tested. Detection of higher concentration samples is done by UV to demonstrate feasibility in this format while lower concentration samples utilize mass spectrometry (MS). Repeatability of successive runs is also tested with particular attention to gradient methods where the equilibration time of the stationary phase can be evaluated. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：102 / 106

页数：5

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