Fecal PCR assay for diagnosis of Helicobacter infection in laboratory rodents

被引：75

作者：

Beckwith, CS

Franklin, CL

Hook, RR

BeschWilliford, CL

Riley, LK

机构：

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1997年 / 35卷 / 06期

关键词：

D O I：

10.1128/JCM.35.6.1620-1623.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A fecal PCR assay for detection of Helicobacter infections in laboratory rodents was developed. DNA was isolated from murine fecal pellets, and a region of the 16S rRNA gene conserved among murine Helicobacter species was amplified. The fecal PCR was sensitive and specific. This assay does not require euthanasia of rodents, which is especially important for valuable rodents, such as transgenic mice.

引用

页码：1620 / 1623

页数：4

共 20 条

[1] DIAGNOSTIC ASSAY FOR HELICOBACTER-HEPATICUS BASED ON NUCLEOTIDE-SEQUENCE OF ITS 16S RIBOSOMAL-RNA GENE
BATTLES, JK
WILLIAMSON, JC
PIKE, KM
GORELICK, PL
WARD, JM
GONDA, MA
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (05) : 1344 - 1347
[2] Blaser M J, 1993, Trends Microbiol, V1, P255, DOI 10.1016/0966-842X(93)90047-U
[3] ORDERED COSMID LIBRARY AND HIGH-RESOLUTION PHYSICAL-GENETIC MAP OF HELICOBACTER-PYLORI STRAIN NCTC11638
BUKANOV, NO
BERG, DE
[J]. MOLECULAR MICROBIOLOGY, 1994, 11 (03) : 509 - 523
[4] HELICOBACTER PYLORI-INDUCED GASTRITIS IN THE DOMESTIC CAT
FOX, JG
BATCHELDER, M
MARINI, R
YAN, L
HANDT, L
LI, X
SHAMES, B
HAYWARD, A
CAMPBELL, J
MURPHY, JC
[J]. INFECTION AND IMMUNITY, 1995, 63 (07) : 2674 - 2681
[5] HELICOBACTER BILIS SP-NOV, A NOVEL HELICOBACTER SPECIES ISOLATED FROM BILE, LIVERS, AND INTESTINES OF AGED, INBRED MICE
FOX, JG
YAN, LL
DEWHIRST, FE
PASTER, BJ
SHAMES, B
MURPHY, JC
HAYWARD, A
BELCHER, JC
MENDES, EN
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (02) : 445 - 454
[6] Chronic proliferative hepatitis in A/JCr mice associated with persistent Helicobacter hepaticus infection: A model of Helicobacter-induced carcinogenesis
Fox, JG
Li, X
Yan, L
Cahill, RJ
Hurley, R
Lewis, R
Murphy, JC
[J]. INFECTION AND IMMUNITY, 1996, 64 (05) : 1548 - 1558
[7] HELICOBACTER HEPATICUS SP-NOV, A MICROAEROPHILIC BACTERIUM ISOLATED FROM LIVERS AND INTESTINAL MUCOSAL SCRAPINGS FROM MICE
FOX, JG
DEWHIRST, FE
TULLY, JG
PASTER, BJ
YAN, L
TAYLOR, NS
COLLINS, MJ
GORELICK, PL
WARD, JM
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (05) : 1238 - 1245
[8] Isolation of a novel Helicobacter species, Helicobacter cholecystus sp nov, from the gallbladders of Syrian hamsters with cholangiofibrosis and centrilobular pancreatitis
Franklin, CL
Beckwith, CS
Livingston, RS
Riley, LK
Gibson, SV
BeschWilliford, CL
Hook, RR
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (12) : 2952 - 2958
[9] HELICOBACTER-MURIDARUM SP-NOV, A MICROAEROPHILIC HELICAL BACTERIUM WITH A NOVEL ULTRASTRUCTURE ISOLATED FROM THE INTESTINAL-MUCOSA OF RODENTS
LEE, A
PHILLIPS, MW
OROURKE, JL
PASTER, BJ
DEWHIRST, FE
FRASER, GJ
FOX, JG
SLY, LI
ROMANIUK, PJ
TRUST, TJ
KOUPRACH, S
[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1992, 42 (01): : 27 - 36
[10] LEE A, 1993, GASTROENTEROL CLIN N, V22, P21

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