Surface localization, regulation, and biologic properties of the 96-kDa alcohol aldehyde dehydrogenase (EhADH2) of pathogenic Entamoeba histolytica

被引：13

作者：

Flores, BM

Stanley, SL

Yong, TS

Ali, M

Yang, WG

Diedrich, DL

Torian, BE

机构：

[1] IDAHO STATE UNIV,COLL PHARM,DEPT PHARMACEUT SCI,POCATELLO,ID 83209

[2] WASHINGTON UNIV,SCH MED,DEPT MED,ST LOUIS,MO 63130

[3] WASHINGTON UNIV,SCH MED,DEPT MOLEC MICROBIOL,ST LOUIS,MO 63130

[4] LOUISIANA STATE UNIV,MED CTR,DEPT MICROBIOL IMMUNOL & PARASITOL,NEW ORLEANS,LA 70112

来源：

JOURNAL OF INFECTIOUS DISEASES | 1996年 / 173卷 / 01期

关键词：

D O I：

10.1093/infdis/173.1.226

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The 96-kDa surface antigen of Entamoeba histolytica was demonstrated through extensive immunologic evaluation with monoclonal and monospecific antibodies to be identical to or an isoform of the amebic alcohol/aldehyde dehydrogenase (EhADH2), EhADH2 was secreted, excreted, or shed into the culture medium in quantities commensurate with amebic growth when studied in a novel culture system. Of importance, using RNase protection assays, specific mRNA coding for the EhADH2 gene product(s) was up-regulated by treatment of viable trophozoites with the enzyme substrate ethanol, These data provide insight into the biology of this enzyme and its regulation by appropriate stressors.

引用

收藏

页码：226 / 231

页数：6

相关论文

共 24 条

[1] PURIFICATION AND MOLECULAR CHARACTERIZATION OF THE NAD(+)-DEPENDENT ACETALDEHYDE ALCOHOL-DEHYDROGENASE FROM ENTAMOEBA-HISTOLYTICA [J].

BRUCHHAUS, I ;

TANNICH, E .

BIOCHEMICAL JOURNAL, 1994, 303 :743-748

[2] THE HEPARIN-BINDING (FIBROBLAST) GROWTH-FACTOR FAMILY OF PROTEINS [J].

BURGESS, WH ;

MACIAG, T .

ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :575-606

[3] EVIDENCE FOR EXPORT OF A MUSCLE LECTIN FROM CYTOSOL TO EXTRACELLULAR-MATRIX AND FOR A NOVEL SECRETORY MECHANISM [J].

COOPER, DNW ;

BARONDES, SH .

JOURNAL OF CELL BIOLOGY, 1990, 110 (05) :1681-1691

[4] NEW MEDIUM FOR AXENIC CULTIVATION OF ENTAMOEBA-HISTOLYTICA AND OTHER ENTAMOEBA [J].

DIAMOND, LS ;

HARLOW, DR ;

CUNNICK, CC .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1978, 72 (04) :431-432

[5] SEROLOGIC REACTIVITY TO PURIFIED RECOMBINANT AND NATIVE 29-KILODALTON PERIPHERAL MEMBRANE-PROTEIN OF PATHOGENIC ENTAMOEBA-HISTOLYTICA [J].

FLORES, BM ;

REED, SL ;

RAVDIN, JI ;

TORIAN, BE .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (06) :1403-1407

[6] CLONING AND SEQUENCE-ANALYSIS OF THE FERMENTATIVE ALCOHOL-DEHYDROGENASE-ENCODING GENE OF ESCHERICHIA-COLI [J].

GOODLOVE, PE ;

CUNNINGHAM, PR ;

PARKER, J ;

CLARK, DP .

GENE, 1989, 85 (01) :209-214

[7] THE MAJOR PARASITE SURFACE-ANTIGEN ASSOCIATED WITH HUMAN RESISTANCE TO SCHISTOSOMIASIS IS A 37-KD GLYCERALDEHYDE-3P-DEHYDROGENASE [J].

GOUDOTCROZEL, V ;

CAILLOL, D ;

DJABALI, M ;

DESSEIN, AJ .

JOURNAL OF EXPERIMENTAL MEDICINE, 1989, 170 (06) :2065-2080

[8] NUCLEOTIDE-SEQUENCE OF A PORPHYROMONAS-GINGIVALIS GENE ENCODING A SURFACE-ASSOCIATED GLUTAMATE-DEHYDROGENASE AND CONSTRUCTION OF A GLUTAMATE DEHYDROGENASE-DEFICIENT ISOGENIC MUTANT [J].

JOE, A ;

MURRAY, CS ;

MCBRIDE, BC .

INFECTION AND IMMUNITY, 1994, 62 (04) :1358-1368

[9] PYRUVATE-FORMATE-LYASE-DEACTIVASE AND ACETYL-COA REDUCTASE ACTIVITIES OF ESCHERICHIA-COLI RESIDE ON A POLYMERIC PROTEIN PARTICLE ENCODED BY ADHE [J].

KESSLER, D ;

LEIBRECHT, I ;

KNAPPE, J .

FEBS LETTERS, 1991, 281 (1-2) :59-63

[10] SECRETION OF PEPTIDES AND PROTEINS LACKING HYDROPHOBIC SIGNAL SEQUENCES - THE ROLE OF ADENOSINE TRIPHOSPHATE-DRIVEN MEMBRANE TRANSLOCATORS [J].

KUCHLER, K ;

THORNER, J .

ENDOCRINE REVIEWS, 1992, 13 (03) :499-514