Dietary selenium reduces the formation of aberrant crypts in rats administered 3,2′-dimethyl-4-aminobiphenyl

被引:55
作者
Feng, Y [1 ]
Finley, JW
Davis, CD
Becker, WK
Fretland, AJ
Hein, DW
机构
[1] Univ N Dakota, Sch Med & Hlth Sci, Dept Surg, Grand Forks, ND 58202 USA
[2] Univ N Dakota, Sch Med & Hlth Sci, Dept Pharmacol & Toxicol, Grand Forks, ND 58202 USA
[3] USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA
[4] Univ Louisville, Sch Med, Dept Pharmacol & Toxicol, Louisville, KY 40292 USA
关键词
selenium; 3,2 '-dimethyl-4-aminobiphenyl; aromatic amines; colorectal cancer; aberrant crypt foci;
D O I
10.1006/taap.1999.8623
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Human epidemiologic studies suggest that low seleniun status is associated with increased cancer risk and that selenium supplementation is associated with reduction in the incidence of several cancers, including colorectal cancer. Aroma and heterocyclic amine carcinogens are thought to be important in the etiology of human colorectal cancer, but no information is available on the effects of selenium on aromatic amine-induced colon cancer. In order to investigate this effect, aberrant crypt foci (ACF), the putative preneoplastic lesions of colon cancer in humans and rodents, were used as a biomarker to test the hypothesis that selenium supplementation can reduce aromatic amine-induced colon carcinogenesis. Male weanling F344 inbred rats were fed a basal torula yeast selenium-deficient diet supplemented with 0, 0.1, or 2.0 mg selenium/kg diet as selenite, selenate, or selenomethionine (SeMet). Animals were fed the diets for 4 weeks and then administered 1 sc injection/week for 2 weeks of 3,2'-dimethyl-4-aminobiphenyl (DMABP; 100 mg/kg) or vehicle peanut oh). At 12 weeks, the rats were euthanized and the colon and rectum were removed, opened longitudinally, and fixed in 70% ethanol. Glutathione peroxidase activities in erythrocytes and liver cytosol and selenium concentrations in the colon/rectum and kidney increased significantly (p < 0.05) and in a dose-dependent manner with each of the three selenium diets. No ACF were identified in vehicle-treated rats; In DMABP-treated rats, ACF frequencies decreased significantly (p < 0.05) in groups supplemented with 0.1 or 2.0 mg selenium/kg diet as selenite and selenate but not SeMet. There were no significant differences in ACF and aberrant crypts between rats fed 0.1 vs 2.0 mg selenium/kg diet. These results suggest that dietary selenium, depending on chemical form, can reduce aromatic amine-induced colon carcinogenesis. (C) 1999 Academic Press.
引用
收藏
页码:36 / 42
页数:7
相关论文
共 66 条
  • [1] *AM CAN SOC, 1997, CANC FACTS FIG
  • [2] [Anonymous], 1991, PRACTICE ONCOLOGY
  • [3] FORMATION AND PERSISTENCE OF ARYLAMINE DNA ADDUCTS INVIVO
    BELAND, FA
    KADLUBAR, FF
    [J]. ENVIRONMENTAL HEALTH PERSPECTIVES, 1985, 62 (OCT) : 19 - 30
  • [4] OBSERVATION AND QUANTIFICATION OF ABERRANT CRYPTS IN THE MURINE COLON TREATED WITH A COLON CARCINOGEN - PRELIMINARY FINDINGS
    BIRD, RP
    [J]. CANCER LETTERS, 1987, 37 (02) : 147 - 151
  • [5] NUTRITION INTERVENTION TRIALS IN LINXIAN, CHINA - SUPPLEMENTATION WITH SPECIFIC VITAMIN MINERAL COMBINATIONS, CANCER INCIDENCE, AND DISEASE-SPECIFIC MORTALITY IN THE GENERAL-POPULATION
    BLOT, WJ
    LI, JY
    TAYLOR, PR
    GUO, WD
    DAWSEY, S
    WANG, GQ
    YANG, CS
    ZHENG, SF
    GAIL, M
    LI, GY
    YU, Y
    LIU, BQ
    TANGREA, J
    SUN, YH
    LIU, FS
    FRAUMENI, JF
    ZHANG, YH
    LI, B
    [J]. JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1993, 85 (18): : 1483 - 1492
  • [6] BONELLI L, 1998, 6 INT S US SEL TELL
  • [7] HUMAN CYTOCHROME P-450PA (P-450IA2), THE PHENACETIN O-DEETHYLASE, IS PRIMARILY RESPONSIBLE FOR THE HEPATIC 3-DEMETHYLATION OF CAFFEINE AND N-OXIDATION OF CARCINOGENIC ARYLAMINES - (AROMATIC-AMINES HETEROCYCLIC AMINES CARCINOGEN METABOLISM)
    BUTLER, MA
    IWASAKI, M
    GUENGERICH, FP
    KADLUBAR, FF
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (20) : 7696 - 7700
  • [8] Effects of selenium supplementation for cancer prevention in patients with carcinoma of the skin a randomized controlled trial - A randomized controlled trial
    Clark, LC
    Combs, GF
    Turnbull, BW
    Slate, EH
    Chalker, DK
    Chow, J
    Davis, LS
    Glover, RA
    Graham, GF
    Gross, EG
    Krongrad, A
    Lesher, JL
    Park, HK
    Sanders, BB
    Smith, CL
    Taylor, JR
    [J]. JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1996, 276 (24): : 1957 - 1963
  • [9] SELENIUM IN FORAGE CROPS AND CANCER MORTALITY IN UNITED-STATES COUNTIES
    CLARK, LC
    CANTOR, KP
    ALLAWAY, WH
    [J]. ARCHIVES OF ENVIRONMENTAL HEALTH, 1991, 46 (01): : 37 - 42
  • [10] CLARK LC, 1985, FED PROC, V44, P2584