Alpha-lipoic acid protects cardiomyocytes against hypoxia/reoxygenation injury by inhibiting autophagy

被引：50

作者：

Cao, Xueming ^{[1
]}

Chen, Aihua ^{[1
]}

Yang, Pingzhen ^{[1
]}

Song, Xudong ^{[1
]}

Liu, Yingfeng ^{[1
]}

Li, Zhiliang ^{[1
]}

Wang, Xianbao ^{[1
]}

Wang, Lizi ^{[1
]}

Li, Yunpeng ^{[1
]}

机构：

[1] Southern Med Univ, Zhujiang Hosp, Dept Cardiol, Guangzhou, Guangdong, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2013年 / 441卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Alpha-lipoic acid; Cardiomyocytes; Hypoxia/reoxygenation; Autophagy; ISCHEMIA-REPERFUSION INJURY; ISCHAEMIA/REPERFUSION INJURY; HEART; DISEASE; DEATH; REOXYGENATION; EXPRESSION; MECHANISM; TARGET;

D O I：

10.1016/j.bbrc.2013.10.166

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Hypoxia/reoxygenation (H/R) is an important in vitro model for exploring the molecular mechanisms and functions of autophagy during myocardial ischemia/reperfusion (I/R). Alpha-lipoic acid (LA) plays an important role in the etiology of cardiovascular disease. Autophagy is widely implicated in myocardial I/R injury. We assessed the degree of autophagy by pretreatment with LA exposed to H/R in H9c2 cell based on the expression levels of Beclin-1, LC3II/LC3I, and green fluorescent protein-labeled LC3 fusion proteins. Autophagic vacuoles were confirmed in H9c2 cells exposed to H/R using transmission electron microscopy. Our findings indicated that pretreatment with LA inhibited the degree of autophagy in parallel to the enhanced cell survival and decreased total cell death in H9c2 cells exposed to H/R. We conclude that LA protects cardiomyocytes against H/R injury by inhibiting autophagy. (C) 2013 Published by Elsevier Inc.

引用

页码：935 / 940

页数：6

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