Characterization of dynamic processes using deuterium in uniformly 2H, 13C, 15N enriched peptides by MAS solid-state NMR

We present in this paper H-2, C-13 MAS correlation experiments that are performed oil a uniformly H-2, C-13, N-15 labeled sample of Nac-Val, and on the uniformly H-2, N-15 labeled dipeptide Nac-Val-Leu-OH. The experiments involve the measurement of (HT)-H-2, relaxation times at two different magnetic fields, as well as the measurement of the H-2 tensor parameters by evolution of the H-2 chemical shift. The data are interpreted quantitatively to differentiate between different side chain motional models. (c) 2005 Elsevier Inc. All rights reserved.