The temperature-signaling cascade in sponges involves a heat-gated cation channel, abscisic acid, and cyclic ADP-ribose

被引:104
作者
Zocchi, E
Carpaneto, A
Cerrano, C
Bavestrello, G
Giovine, M
Bruzzone, S
Guida, L
Franco, L
Usai, C
机构
[1] Univ Genoa, Sezione Biochim, Dipartimento Med Sperimentale, I-16132 Genoa, Italy
[2] CNR, Inst Cybernet & Biophys, I-16149 Genoa, Italy
[3] Univ Genoa, Dipartimento Studio Territorio & Sue Risorse, I-16132 Genoa, Italy
[4] Univ Ancona, Ist Sci Mare, I-60131 Ancona, Italy
关键词
D O I
10.1073/pnas.261448698
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Sponges (phylum Porifera) are the phylogenetically oldest metazoan animals, their evolution dating back to 600 million years ago. Here we demonstrate that sponges express ADP-ribosyl cyclase activity, which converts NAD(+) into cyclic ADP-ribose, a potent and universal intracellular Ca2+ mobilizer. In Axinella polypoides (Demospongiae, Axinellidae), ADP-ribosyl cyclase was activated by temperature increases by means of an abscisic acid-induced, protein kinase A-dependent mechanism. The thermosensor triggering this signaling cascade was a heat-activated cation channel. Elucidation of the complete thermosensing pathway in sponges highlights a number of features conserved in higher organisms: (t) the cation channel thermoreceptor, sensitive to heat, mechanical stress, phosphorylation, and anesthetics, shares all of the functional characteristics of the mammalian heat-activated background K+ channel responsible for central and peripheral thermosensing; (it) involvement of the phytohormone abscisic acid and cyclic ADP-ribose as its second messenger is reminiscent of the drought stress signaling pathway in plants. These results suggest an ancient evolutionary origin of this stress-signaling cascade in a common precursor of modern Metazoa and Metaphyta.
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页码:14859 / 14864
页数:6
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