Isolation of Rickettsia felis in the mosquito cell line C6/36

被引：39

作者：

Horta, MC

Labruna, MB ^{[1
]}

Durigon, EL

Schumaker, TTS

机构：

[1] Univ Sao Paulo, Fac Med Vet, Dept Med Vet Prevent & Saude Anim, Lab Doencas Parasitarias, BR-05508270 Sao Paulo, Brazil

[2] Univ Sao Paulo, Dept Microbiol, BR-05508900 Sao Paulo, Brazil

[3] Univ Sao Paulo, Dept Parasitol, BR-05508900 Sao Paulo, Brazil

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2006年 / 72卷 / 02期

关键词：

D O I：

10.1128/AEM.72.2.1705-1707.2006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report the isolation and establishment of Rickettsia felis in the C6/36 cell line. Rickettsiall growth was intense, always with 90 to 100% of cells being infected after few weeks. The rickettsial isolate was confirmed by testing infected cells by PCR and sequencing fragments of three major Rickettsia genes (gltA, ompB, and the 17-kDa protein gene).

引用

页码：1705 / 1707

页数：3

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