Understanding the Antibacterial Mechanism of CuO Nanoparticles: Revealing the Route of Induced Oxidative Stress

被引：476

作者：

Applerot, Guy ^{[1
,2
]}

Lellouche, Jonathan ^{[1
,2
,3
]}

Lipovsky, Anat ^{[1
,2
]}

Nitzan, Yeshayahu ^{[3
]}

Lubart, Rachel ^{[1
,2
]}

Gedanken, Aharon ^{[1
,2
]}

Banin, Ehud ^{[3
]}

机构：

[1] Bar Ilan Univ, Ctr Adv Mat & Nanotechnol, Dept Chem, IL-52900 Ramat Gan, Israel

[2] Bar Ilan Univ, Ctr Adv Mat & Nanotechnol, Kanbar Lab Nanomat, IL-52900 Ramat Gan, Israel

[3] Bar Ilan Univ, Ctr Adv Mat & Nanotechnol, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel

来源：

SMALL | 2012年 / 8卷 / 21期

关键词：

biomedical materials; CuO; sonochemistry; reactive oxygen species; nanoparticles; COPPER-OXIDE NANOPARTICLES; SILVER NANOPARTICLES; ZNO NANOPARTICLES; TOXICITY; NANOMATERIALS; NANOTUBES; MICROWAVE; RADICALS; SUICIDE; SYSTEMS;

D O I：

10.1002/smll.201200772

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

To date, there is still a lack of definite knowledge regarding the interaction of CuO nanoparticles with bacteria and the possible permeation of the nanoparticles into bacterial cells. This study was aimed at shedding light on the size-dependent (from the microscale down to the small nanoscale) antibacterial activity of CuO. The potent antibacterial activity of CuO nanoparticles was found to be due to ROS-generation by the nanoparticles attached to the bacterial cells, which in turn provoked an enhancement of the intracellular oxidative stress. This paradigm was confirmed by several assays such as lipid peroxidation and reporter strains of oxidative stress. Furthermore, electron microscopy indicated that the small nanoparticles of CuO penetrated the cells. Collectively, the results reported herein may reconcile conflicting concepts in the literature concerning the antibacterial mechanism of CuO nanoparticles, as well as highlight the potential for developing sustainable CuO nanoparticles-based devices for inhibiting bacterial infections.

引用

页码：3326 / 3337

页数：12

共 61 条

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