Caenorhabditis elegans mutant allele identification by whole-genome sequencing

被引：169

作者：

Sarin, Sumeet ^{[2
]}

Prabhu, Snehit ^{[1
]}

O'Meara, M. Maggie ^{[2
]}

Pe'er, Itsik ^{[1
]}

Hobert, Oliver ^{[2
]}

机构：

[1] Columbia Univ, Dept Comp Sci, New York, NY 10027 USA

[2] Columbia Univ, Howard Hughes Med Inst, Med Ctr, Dept Biochem & Mol Biophys, New York, NY 10032 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 10期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/NMETH.1249

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Identification of the molecular lesion in Caenorhabditis elegans mutants isolated through forward genetic screens usually involves time-consuming genetic mapping. We used Illumina deep sequencing technology to sequence a complete, mutant C. elegans genome and thus pinpointed a single-nucleotide mutation in the genome that affects a neuronal cell fate decision. This constitutes a proof-of-principle for using whole-genome sequencing to analyze C. elegans mutants.

引用

页码：865 / 867

页数：3

共 7 条

[1] Whole-genome re-sequencing [J].

Bentley, David R. .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 2006, 16 (06) :545-552

[2]

BRENNER S, 1974, GENETICS, V77, P71

[3] A transcriptional regulatory cascade that controls left/right asymmetry in chemosensory neurons of C-elegans [J].

Chang, S ;

Johnston, RJ ;

Hobert, O .

GENES & DEVELOPMENT, 2003, 17 (17) :2123-2137

[4] Rapid single nucleotide polymorphism mapping in C-elegans -: art. no. 118 [J].

Davis, MW ;

Hammarlund, M ;

Harrach, T ;

Hullett, P ;

Olsen, S ;

Jorgensen, EM .

BMC GENOMICS, 2005, 6 (1)

[5] Whole-genome sequencing and variant discovery in C-elegans [J].

Hillier, LaDeana W. ;

Marth, Gabor T. ;

Quinlan, Aaron R. ;

Dooling, David ;

Fewell, Ginger ;

Barnett, Derek ;

Fox, Paul ;

Glasscock, Jarret I. ;

Hickenbotham, Matthew ;

Huang, Weichun ;

Magrini, Vincent J. ;

Richt, Ryan J. ;

Sander, Sacha N. ;

Stewart, Donald A. ;

Stromberg, Michael ;

Tsung, Eric F. ;

Wylie, Todd ;

Schedl, Tim ;

Wilson, Richard K. ;

Mardis, Elaine R. .

NATURE METHODS, 2008, 5 (02) :183-188

[6]

LANDER E S, 1988, Genomics, V2, P231

[7] Genetic screens for Caenorhabditis elegans mutants defective in left/right asymmetric neuronal fate specification [J].

Sarin, Sumeet ;

O'Meara, M. Maggie ;

Flowers, Eileen B. ;

Antonio, Celia ;

Poole, Richard J. ;

Didiano, Dominic ;

Johnston, Robert J., Jr. ;

Chang, Sarah ;

Narula, Surinder ;

Hobert, Oliver .

GENETICS, 2007, 176 (04) :2109-2130

← 1 →