Plant-produced hepatitis B core protein chimera carrying anthrax protective antigen domain-4

被引：10

作者：

Bandurska, Katarzyna ^{[1
]}

Brodzik, Robert ^{[1
]}

Spitsin, Sergei ^{[1
]}

Kohl, Thomas ^{[1
]}

Portocarrero, Carla ^{[1
]}

Smirnov, Yuri ^{[1
]}

Pogrebnyak, Natalia ^{[1
]}

Sirko, Agnieszka ^{[2
]}

Koprowski, Hilary ^{[1
]}

Golovkin, Maxim ^{[1
]}

机构：

[1] Thomas Jefferson Univ, Biotechnol Fdn Labs, Philadelphia, PA 19107 USA

[2] Polish Acad Sci, Inst Biochem & Biophys, Warsaw, Poland

来源：

HYBRIDOMA | 2008年 / 27卷 / 04期

关键词：

D O I：

10.1089/hyb.2008.0008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The hepatitis B core antigen (HBcAg) can generate a strong immune response and is recognized as an effective carrier for foreign epitopes. The domain-4 epitope of the anthrax protective antigen (PA-D4) plays an essential role in generating protective immunity against virulent Bacillus anthracis. Here we report the successful production of a recombinant protein comprised of the antigenic PA-D4 integrated into the c/e1 loop of HBcAg in transgenic low-alkaloid Nicotiana tabacum. Sera of mice injected with the plant-derived purified HB/PA-D4 protein exhibited significant anti-PA- and anti-HBcAg-specific IgG titers; however, formation of virus-like particles (VLP) was not observed. These data support the feasibility of producing complex protein chimeras in plants.

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收藏

页码：241 / 247

页数：7

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