The presence of simian-virus 40 sequences in mesothelioma and mesothelial cells is associated with high levels of vascular endothelial growth factor

被引:45
作者
Cacciotti, P
Strizzi, L
Vianale, G
Iaccheri, L
Libener, R
Porta, C
Tognon, M
Gaudino, G
Mutti, L
机构
[1] Univ Piemonte Orientale, Dept Med Sci, Novara, Italy
[2] Univ G DAnnunzio, Dept Oncol & Neurosci, Chieti, Italy
[3] IRCSS Maugeri Fdn, Pavia, Italy
[4] ASL 11, Piemonte, Italy
[5] Univ Ferrara, Dept Morphol & Embryol, Sect Histol & Embryol, I-44100 Ferrara, Italy
[6] SS Antonio & Biagio Hosp, Pathol Serv, Alessandria, Italy
[7] San Matteo Univ Hosp, IRCCS, Pavia, Italy
关键词
D O I
10.1165/ajrcmb.26.2.4673
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The aim of this study was to evaluate whether the presence of simian virus-40 (SV40) is associated with increased release of vascular endothelial growth factor (VEGF) in human malignant mesothelioma (MM) cells. We studied nine cell lines derived from pleural effusion (PE) of patients with MM, and three different cultures of normal human mesothelial cells (NHMC) derived from pleural fluid of patients with congestive heart failure. NHMC were transfected with full length SV40 (NHMC-FL) or large T antigen (NHMC Tag) DNAs. High levels of VEGF were detected in conditioned media of each of two MM cells that tested positive for SV40 by PCR amplification and Southern blot hybridization and for Tag transcript by reverse transcription-polymerase chain reaction (RT-PCR) and immunoprecipitation. We also found that NHMC-FL released high amounts of VEGF. Conditioned media from SV40-positive MM cells and from FL-NHMC increased proliferation of human umbilical vein cells (HUVEC) and this effect was partially abrogated by adding specific blocking antibodies against VEGF. These results offer the first evidence that SV40 can cause VEGF release in SV40-positive MM cells and that entire viral genome is required for this effect.
引用
收藏
页码:189 / 193
页数:5
相关论文
共 37 条
[1]   NATURAL-HISTORY AND EPIDEMIOLOGY OF MALIGNANT MESOTHELIOMA [J].
ANTMAN, KH .
CHEST, 1993, 103 (04) :S373-S376
[2]  
ASPLUND T, 1993, CANCER RES, V53, P388
[3]   Human mesothelial cells are unusually susceptible to simian virus 40-mediated transformation and asbestos cocarcinogenicity [J].
Bocchetta, M ;
Di Resta, I ;
Powers, A ;
Fresco, R ;
Tosolini, A ;
Testa, JR ;
Pass, HI ;
Rizzo, P ;
Carbone, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (18) :10214-10219
[4]   Malignant pleural mesothelioma [J].
Boutin, C ;
Schlesser, M ;
Frenay, C ;
Astoul, P .
EUROPEAN RESPIRATORY JOURNAL, 1998, 12 (04) :972-981
[5]   INDUCTION OF ANGIOGENESIS BY INTRAPERITONEAL INJECTION OF ASBESTOS FIBERS [J].
BRANCHAUD, RM ;
MACDONALD, JL ;
KANE, AB .
FASEB JOURNAL, 1989, 3 (06) :1747-1752
[6]   HEPATOCYTE GROWTH-FACTOR IS A POTENT ANGIOGENIC FACTOR WHICH STIMULATES ENDOTHELIAL-CELL MOTILITY AND GROWTH [J].
BUSSOLINO, F ;
DIRENZO, MF ;
ZICHE, M ;
BOCCHIETTO, E ;
OLIVERO, M ;
NALDINI, L ;
GAUDINO, G ;
TAMAGNONE, L ;
COFFER, A ;
COMOGLIO, PM .
JOURNAL OF CELL BIOLOGY, 1992, 119 (03) :629-641
[7]  
CARBONE M, 1994, ONCOGENE, V9, P1781
[8]  
Carbone M, 1999, J CELL PHYSIOL, V180, P167, DOI 10.1002/(SICI)1097-4652(199908)180:2<167::AID-JCP4>3.0.CO
[9]  
2-Q
[10]   Simian virus-40 large-T antigen binds p53 in human mesotheliomas [J].
Carbone, M ;
Rizzo, P ;
Grimley, PM ;
Procopio, A ;
Mew, DJY ;
Shridhar, V ;
DeBartolomeis, A ;
Esposito, V ;
Giuliano, MT ;
Steinberg, SM ;
Levine, AS ;
Giordano, A ;
Pass, HI .
NATURE MEDICINE, 1997, 3 (08) :908-912