Properties of phenoloxidases generated from prophenoloxidase with 2-propanol and the natural activator in Drosophila melanogaster

被引：26

作者：

Asada, N ^{[1
]}

Sezaki, H

机构：

[1] Okayama Univ Sci, Fac Sci, Biol Lab, Okayama 7000005, Japan

[2] Osaka Univ, Inst Prot Res, Div Prot Chem, Suita, Osaka 5650871, Japan

来源：

BIOCHEMICAL GENETICS | 1999年 / 37卷 / 5-6期

关键词：

Drosophila; prophenoloxidase; activation; kinetics;

D O I：

10.1023/A:1018730404305

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Prophenoloxidases A(1) and A(3) in Drosophila melanogaster were activated with 2-propanol and a partially purified natural activator For prophenoloxidase activation the optimum temperature was 30 degrees C and the optimum pH was 8. Both mono- and diphenoloxidase activities were found in A(1) and A(3) activated with 2-propanol, whereas only diphenoloxidase activity was detected in A(3) activated with a natural activator The kinetic properties, K-m and V-max, were not similar in those phenoloxidases activated with different activating agents. The rate of inhibition of phenoloxidase by diethyldithiocarbamate and phenylthiocarbamate depended on the concentration of 2-propanol. Both compounds exhibited a noncompetitive pattern of inhibition.

引用

页码：149 / 158

页数：10

共 20 条

[1] ACTIVATION OF PROPHENOLOXIDASE WITH 2-PROPANOL AND OTHER ORGANIC-COMPOUNDS IN DROSOPHILA-MELANOGASTER
ASADA, N
FUKUMITSU, T
FUJIMOTO, K
MASUDA, K
[J]. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1993, 23 (04) : 515 - 520
[2] Asada N, 1998, J EXP ZOOL, V282, P28, DOI 10.1002/(SICI)1097-010X(199809/10)282:1/2<28::AID-JEZ6>3.3.CO
[3] 2-U
[4] Ashida M., 1990, P239
[5] CHARACTERIZATION OF HEMOLYMPH PROTYROSINASE AND A CUTICULAR ACTIVATOR FROM MANDUCA-SEXTA (L)
ASO, Y
KRAMER, KJ
HOPKINS, TL
LOOKHART, GL
[J]. INSECT BIOCHEMISTRY, 1985, 15 (01): : 9 - +
[6] ASO Y, 1984, INSECT BIOCHEM, V14, P463, DOI 10.1016/0020-1790(84)90103-3
[7] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[8] Activation of prophenoloxidase A(1) by an activating enzyme in Drosophila melanogaster
Chosa, N
Fukumitsu, T
Fujimoto, K
Ohnishi, E
[J]. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1997, 27 (01) : 61 - 68
[9] DUCKWORTH HW, 1970, J BIOL CHEM, V245, P1613
[10] PURIFICATION AND CHARACTERIZATION OF PROPHENOLOXIDASES FROM PUPAE OF DROSOPHILA-MELANOGASTER
FUJIMOTO, K
MASUDA, K
ASADA, N
OHNISHI, E
[J]. JOURNAL OF BIOCHEMISTRY, 1993, 113 (03) : 285 - 291

← 1 2 →