Properties of phenoloxidases generated from prophenoloxidase with 2-propanol and the natural activator in Drosophila melanogaster

被引:26
作者
Asada, N [1 ]
Sezaki, H
机构
[1] Okayama Univ Sci, Fac Sci, Biol Lab, Okayama 7000005, Japan
[2] Osaka Univ, Inst Prot Res, Div Prot Chem, Suita, Osaka 5650871, Japan
关键词
Drosophila; prophenoloxidase; activation; kinetics;
D O I
10.1023/A:1018730404305
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Prophenoloxidases A(1) and A(3) in Drosophila melanogaster were activated with 2-propanol and a partially purified natural activator For prophenoloxidase activation the optimum temperature was 30 degrees C and the optimum pH was 8. Both mono- and diphenoloxidase activities were found in A(1) and A(3) activated with 2-propanol, whereas only diphenoloxidase activity was detected in A(3) activated with a natural activator The kinetic properties, K-m and V-max, were not similar in those phenoloxidases activated with different activating agents. The rate of inhibition of phenoloxidase by diethyldithiocarbamate and phenylthiocarbamate depended on the concentration of 2-propanol. Both compounds exhibited a noncompetitive pattern of inhibition.
引用
收藏
页码:149 / 158
页数:10
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