Permeability of large unilamellar digalactosyldiacylglycerol vesicles for protons and glucose -: influence of α-tocopherol, β-carotene, zeaxanthin and cholesterol

被引:35
作者
Berglund, AH [1 ]
Nilsson, R [1 ]
Liljenberg, C [1 ]
机构
[1] Gothenburg Univ, Inst Bot, Dept Plant Physiol, S-40530 Gothenburg, Sweden
关键词
carboxyfluorescein; galactolipid; model membranes; pyranine; thylakoid;
D O I
10.1016/S0981-9428(99)80032-1
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The permeability of large unilamellar vesicles formed from digalactosyldiacylglycerol for glucose and protons was measured. The vesicle composition was modified by addition of different terpenoids: cc-tocopherol, cholesterol, zeaxanthin and B-carotene. The digalactosyldiacylglycerol species composition was dominated by the species 18:2/18:2 and 18:1/18:2. Using the self-quenching properties of the fluorescent probe 6-carboxyfluorescein trapped in the aqueous space of the vesicles, the permeability for glucose was determined with a glucose gradient of 800 mM. The calculated permeability coefficient for glucose was in the range of 4.85(.)10(-10) to 1.12.10(-9) cm.S-1. For proton permeability measurements, the pH-sensitive fluorescent dye pyranine was used. The proton permeability was measured with a pH gradient of 0.6 pH units from 7.0 to 7.6 with valinomycin present to dissipate any diffusion potential across the membrane. The permeability coefficients for protons were in the range of 3.5.10(-8) to 1.0(.)10(-7) cm s(-1). Digalactosyldiacylglycerol vesicles with 5 % alpha-tocopherol or 10 % cholesterol or 2 % zeaxanthin reduced the permeability for protons, the two latter significantly as compared to digalactosyldiacylglycerol vesicles. alpha-Tocopherol (5 %) decreased the permeability for glucose remarkably and so did cholesterol (10 %). B-carotene (< than 1 %) and zeaxanthin (2 %) in galactolipid vesicles, however, increased the permeability The significance of these results is discussed in relation to the physiological functions of galactolipids and terpenoids in chloroplast membranes. (C) Elsevier, Paris.
引用
收藏
页码:179 / 186
页数:8
相关论文
共 36 条
[1]   PLANT MEMBRANE LIPIDS [J].
BENSON, AA .
ANNUAL REVIEW OF PLANT PHYSIOLOGY, 1964, 15 :1-&
[2]   ION AND SUGAR PERMEABILITIES OF LECITHIN BILAYERS - COMPARISON OF CURVED AND PLANAR BILAYERS [J].
BRUNNER, J ;
GRAHAM, DE ;
HAUSER, H ;
SEMENZA, G .
JOURNAL OF MEMBRANE BIOLOGY, 1980, 57 (02) :133-141
[3]   PYRANINE (8-HYDROXY-1,3,6-PYRENETRISULFONATE) AS A PROBE OF INTERNAL AQUEOUS HYDROGEN-ION CONCENTRATION IN PHOSPHOLIPID-VESICLES [J].
CLEMENT, NR ;
GOULD, JM .
BIOCHEMISTRY, 1981, 20 (06) :1534-1538
[4]   PROTON HYDROXIDE PERMEABILITY OF LIPOSOMES [J].
DEAMER, DW ;
NICHOLS, JW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (01) :165-168
[5]   PROTON FLUX MECHANISMS IN MODEL AND BIOLOGICAL-MEMBRANES [J].
DEAMER, DW ;
NICHOLS, JW .
JOURNAL OF MEMBRANE BIOLOGY, 1989, 107 (02) :91-103
[6]  
DEAMER DW, 1987, J BIOENERG BIOMEMBR, V19, P457
[7]   THE ANTIOXIDANT EFFECTS OF THYLAKOID VITAMIN-E(ALPHA-TOCOPHEROL) [J].
FRYER, MJ .
PLANT CELL AND ENVIRONMENT, 1992, 15 (04) :381-392
[8]   PERMEABILITY AND ELECTRICAL-PROPERTIES OF PLANAR LIPID-MEMBRANES FROM THYLAKOID LIPIDS [J].
FUKS, B ;
HOMBLE, F .
BIOPHYSICAL JOURNAL, 1994, 66 (05) :1404-1414
[9]   Mechanism of proton permeation through chloroplast lipid membranes [J].
Fuks, B ;
Homble, F .
PLANT PHYSIOLOGY, 1996, 112 (02) :759-766
[10]   Zeaxanthin (dihydroxy-beta-carotene but not beta-carotene rigidifies lipid membranes: A H-1-NMR study of carotenoid-egg phosphatidylcholine liposomes [J].
Gabrielska, J ;
Gruszecki, WI .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1996, 1285 (02) :167-174