Large-scale production of N-acetyllactosamine through bacterial coupling

被引：52

作者：

Endo, T ^{[1
]}

Koizumi, S ^{[1
]}

Tabata, K ^{[1
]}

Kakita, S ^{[1
]}

Ozaki, A ^{[1
]}

机构：

[1] Kyowa Hakko Kogyo Co Ltd, Tokyo Res Labs, Machida, Tokyo 1948533, Japan

来源：

CARBOHYDRATE RESEARCH | 1999年 / 316卷 / 1-4期

关键词：

enzymatic synthesis; glycosyltransferase; sugar nucleotide; oligosaccharide;

D O I：

10.1016/S0008-6215(99)00050-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A large-scale production system of N-acetyllactosamine, a core structure of various oligosaccharides, was established by a whole-cell reaction through the combination of recombinant Escherichia coli strains and Corynebacterium ammoniagenes. Two recombinant E. coli strains over-expressed the UDP-Gal biosynthetic genes and the beta-(1 --> 4)-galactosyltransferase gene of Neisseria gonorrhoeae, respectively. C. ammoniagenes contributed the production of UTP from orotic acid. N-Acetyllactosamine was accumulated at 279 mM (107 g L-1) after a 38 h reaction (2.5 L in volume) starting from orotic acid, D-galactose, and 2-acetamido-2-deoxy-D-glucose. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：179 / 183

页数：5

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