Evidence for a mediator cycle at the initiation of transcription

被引：102

作者：

Svejstrup, JQ ^{[1
]}

Li, Y ^{[1
]}

Fellows, J ^{[1
]}

Gnatt, A ^{[1
]}

Bjorklund, S ^{[1
]}

Kornberg, RD ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND,CLARE HALL LABS,S MIMMS EN6 3LD,HERTS,ENGLAND

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1997年 / 94卷 / 12期

关键词：

RNA polymerase II; C-terminal domain; yeast;

D O I：

10.1073/pnas.94.12.6075

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Free and elongating (DNA-bound) forms of RNA polymerase II were separated from yeast. Most cellular polymerase II was found in the elongating fraction, which contained all enzyme phosphorylated on the C-terminal domain and none of the 15-subunit mediator of transcriptional regulation, These and other findings suggest that mediator enters and leaves initiation complexes during every round of transcription, in a process that may be coupled to C-terminal domain phosphorylation.

引用

页码：6075 / 6078

页数：4

共 19 条

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