Electron energy loss spectroscopy techniques for the study of microbial chromium(VI) reduction

被引:85
作者
Daulton, TL
Little, BJ [1 ]
Lowe, K
Jones-Meehan, J
机构
[1] USN, Res Lab, Div Oceanog, Stennis Space Ctr, MS 39529 USA
[2] USN, Res Lab, Marine Geosci Div, Stennis Space Ctr, MS 39529 USA
[3] USN, Res Lab, Div Chem, Washington, DC 20375 USA
关键词
Cr(VI); electron energy loss spectroscopy; environmental cell; microbial metal reduction; Shewanella oneidensis; transmission electron microscopy;
D O I
10.1016/S0167-7012(02)00013-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Electron energy loss spectroscopy (EELS) techniques were used to determine oxidation state, at high spatial resolution. of chromium associated with the metal-reducing bacteria, Shewanella oneidensis, in anaerobic cultures containing Cr(VI)O-4(2-). These techniques were applied to fixed cells examined in thin section by conventional transmission electron microscopy (TEM) as well as unfixed, hydrated bacteria examined by environmental cell (EC)-TEM. Two distinct populations of bacteria were observed by TEM: bacteria exhibiting low image contrast and bacteria exhibiting high contrast in their cell membrane (or boundary) structure which was often encrusted with high-contrast precipitates. Measurements by EELS demonstrated that cell boundaries became saturated with low concentrations of Cr and the precipitates encrusting bacterial cells contained a reduced form of Cr in oxidation state +3 or lower. Published by Elsevier Science B.V.
引用
收藏
页码:39 / 54
页数:16
相关论文
共 52 条
[1]  
ALLEN GC, 1973, J CHEM SOC DALTON, P1675
[2]   MULTIPLET SPLITTING OF X-RAY PHOTOELECTRON LINES OF CHROMIUM COMPLEXES - EFFECT OF COVALENCY ON 2P CORE LEVEL SPIN-ORBIT SEPARATION [J].
ALLEN, GC ;
TUCKER, PM .
INORGANICA CHIMICA ACTA, 1976, 16 (JAN) :41-45
[3]   X-RAY PHOTOELECTRON-SPECTRA OF SEVERAL OXIDES OF IRON AND CHROMIUM [J].
ASAMI, K ;
HASHIMOTO, K .
CORROSION SCIENCE, 1977, 17 (07) :559-570
[4]   Reduction of chromate by microorganisms isolated from metal contaminated sites of Karachi, Pakistan [J].
Badar, U ;
Ahmed, N ;
Beswick, AJ ;
Pattanapipitpaisal, P ;
Macaskie, LE .
BIOTECHNOLOGY LETTERS, 2000, 22 (10) :829-836
[5]   Equilibria of chromate(VI) species in acid medium and ab initio studies of these species [J].
Brito, F ;
Ascanio, J ;
Mateo, S ;
Hernandez, C ;
Araujo, L ;
Gili, P ;
MartinZarza, P ;
Dominguez, S ;
Mederos, A .
POLYHEDRON, 1997, 16 (21) :3835-3846
[6]   L2,3 EDGES OF TETRAHEDRALLY COORDINATED D(0) TRANSITION-METAL OXYANIONS XO4N- [J].
BRYDSON, R ;
GARVIE, LAJ ;
CRAVEN, AJ ;
SAUER, H ;
HOFER, F ;
CRESSEY, G .
JOURNAL OF PHYSICS-CONDENSED MATTER, 1993, 5 (50) :9379-9392
[7]   BACTERIAL INTERACTIONS WITH CHROMATE [J].
CERVANTES, C .
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 1991, 59 (04) :229-233
[8]  
Cervantes C, 2001, FEMS MICROBIOL REV, V25, P335, DOI 10.1111/j.1574-6976.2001.tb00581.x
[9]   Toxic and mutagenic effects of chromium(VI). A review [J].
CieslakGolonka, M .
POLYHEDRON, 1996, 15 (21) :3667-3689
[10]  
Clesceri LS., 1999, STANDARD METHODS EXA