Positioning of two alpha subunit carboxy-terminal domains of RNA polymerase at promoters by two transcription factors

被引：77

作者：

Murakami, K

Owens, JT

Belyaeva, TA

Meares, CF

Busby, SJW

Ishihama, A

机构：

[1] NATL INST GENET,DEPT MOL GENET,MISHIMA,SHIZUOKA 411,JAPAN

[2] GRAD UNIV ADV STUDIES,SCH LIFE SCI,MISHIMA,SHIZUOKA 411,JAPAN

[3] UNIV CALIF DAVIS,DEPT CHEM,DAVIS,CA 95616

[4] UNIV BIRMINGHAM,SCH BIOCHEM,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1997年 / 94卷 / 21期

基金：

英国惠康基金;

关键词：

protein-protein contact; protein-DNA contact; chemical nuclease; transcription regulation;

D O I：

10.1073/pnas.94.21.11274

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Interactions between the cAMP receptor protein (CRP) and the carboxy-terminal regulatory domain (CTD) of Escherichia coli RNA polymerase alpha subunit were analyzed at promoters carrying tandem DNA sites for CRP binding using a chemical nuclease covalently attached to alpha. Each CRP dimer was found to direct the positioning of one of the two alpha subunit CTDs. Thus, the function of RNA polymerase may be subject to regulation through protein-protein interactions between the two (alpha subunits and two different species of transcription factors.

引用

页码：11274 / 11278

页数：5

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