Isolation of human hepatocytes after hepatic warm and cold ischemia: A practical approach using University of Wisconsin solution

被引：6

作者：

Caruana, M

Battle, T

Fuller, B

Davidson, B

机构：

[1] UCL Royal Free & Univ Coll Med Sch, Dept Surg, London NW3 2QG, England

[2] Publ Hlth Lab Serv, Ctr Appl Microbiol & Res, Salisbury SP4 0JG, Wilts, England

[3] UCL Royal Free & Univ Coll Med Sch, Liver Transplant Unit, London NW3 2QG, England

来源：

CRYOBIOLOGY | 1999年 / 38卷 / 02期

关键词：

D O I：

10.1006/cryo.1999.2155

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A method is described for isolating human hepatocytes from tissue fragments after warm and cold ischemia as experienced during hepatic resections. Cells with a high trypan blue dye exclusion and good culture characteristics were isolated by employing an initial tissue perfusion with UW solution. The method could facilitate transfer of liver tissues between distant centers for cell isolation studies, (C) 1999 Academic Press.

引用

页码：165 / 168

页数：4

共 7 条

[1] ISOLATION, CULTURE AND CHARACTERIZATION OF ADULT HUMAN HEPATOCYTES FROM SURGICAL LIVER BIOPSIES [J].