Lipoprotein is a predominant Toll-like receptor 2 ligand in Staphylococcus aureus cell wall components

被引:136
作者
Hashimoto, M
Tawaratsumida, K
Kariya, H
Aoyama, K
Tamura, T
Suda, Y
机构
[1] Kagoshima Univ, Dept Nanostruct & Adv Mat, Kagoshima 8900065, Japan
[2] Hyogo Med Univ, Dept Microbiol, Nishinomiya, Hyogo 6638501, Japan
关键词
innate immunity; lipoteichoic acid; PAMPs; TLR; Triton X-114;
D O I
10.1093/intimm/dxh374
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Lipoteichoic acid (LTA) derived from Staphylococcus aureus is reported to be a ligand of Toll-like receptor 2 (TLR2). In this study, we demonstrated that lipoproteins obtained from S. aureus are potent activators of TLR2. A fraction obtained by Triton X-114 phase partitioning activated cells through TLR2. The fraction contained proteins and LTA. The activity was detected in compounds in a mass range of 12-40 kDa. Proteinase K digested the active compounds into lower molecular weight active materials < 10 kDa. In contrast, hydrofluoric acid treatment, which decomposes LTA, did not alter the molecular mass of the active compounds. Further, most of the activity was abrogated by lipoprotein lipase digestion. These results suggested that lipoproteins are predominant TLR2 ligands in S. aureus cell wall components.
引用
收藏
页码:355 / 362
页数:8
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