Glycan microarray analysis of the hemagglutinins from modern and pandemic influenza viruses reveals different receptor specificities

被引:502
作者
Stevens, J
Blixt, O
Glaser, L
Taubenberger, JK
Palese, P
Paulson, JC
Wilson, IA
机构
[1] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA
[2] Scripps Res Inst, Glycan Array Synth Core D, Consortium Funct Glycom, La Jolla, CA 92037 USA
[3] CUNY Mt Sinai Sch Med, Dept Microbiol, New York, NY 10029 USA
[4] Armed Forces Inst Pathol, Dept Mol Pathol, Washington, DC 20306 USA
[5] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA
关键词
glycoarray; influenza; 1918; pandemic; hemagglutinin; receptor specificity;
D O I
10.1016/j.jmb.2005.11.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Influenza A virus specificity for the host is mediated by the viral surface glycoprotein hemagglutinin (HA), which binds to receptors containing glycans with terminal sialic acids. Avian viruses preferentially bind to alpha 2-3-linked sialic acids on receptors of intestinal epithelial cells, whereas human viruses are specific for the alpha 2-6 linkage on epithelial cells of the lungs and upper respiratory tract. To define the receptor preferences of a number of human and avian H1 and H3 viruses, including the 1918 H1N1 pandemic strains, their hemagglutinins were analyzed using a recently described glycan array. The array, which contains 200 carbohydrates and glycoproteins, not only revealed clear differentiation of receptor preferences for alpha 2-3 and/or alpha 2-6 sialic acid linkage, but could also detect fine differences in HA specificity, such as preferences for fucosylation, sulfation and sialylation at positions 2 (Gal) and 3 (GlcNAc, GalNAc) of the terminal trisaccharide. For the two 1918 HA variants, the South Carolina (SC) HA (with Asp190, Asp225) bound exclusively alpha 2-6 receptors, while the New York (NY) variant, which differed only by one residue (Gly225), had mixed alpha 2-6/alpha 2-3 specificity, especially for sulfated oligosaccharides. Only one mutation of the NY variant (Asp190Glu) was sufficient to revert the HA receptor preference to that of classical avian strains. Thus, the species barrier, as defined by the receptor specificity preferences of 1918 human viruses compared to likely avian virus progenitors, can be circumvented by changes at only two positions in the HA receptor binding site. The glycan array thus provides highly detailed profiles of influenza receptor specificity that can be used to map the evolution of new human pathogenic strains, such as the H5N1 avian influenza. (c) Elsevier Ltd. All rights reserved.
引用
收藏
页码:1143 / 1155
页数:13
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