Cross-Priming Amplification for Rapid Detection of Mycobacterium tuberculosis in Sputum Specimens

被引：140

作者：

Fang, Rendong ^{[2
,3
]}

Li, Xia ^{[2
]}

Hu, Lin ^{[4
]}

You, Qimin ^{[4
]}

Li, Jing ^{[3
]}

Wu, Jie ^{[3
]}

Xu, Peng ^{[2
,3
]}

Zhong, Huayan ^{[4
]}

Luo, Ying ^{[4
]}

Mei, Jian ^{[3
]}

Gao, Qian ^{[1
,2
]}

机构：

[1] Fudan Univ, Shanghai Med Coll, Key Lab Med Mol Virol, Inst Biomed Sci, Shanghai 200032, Peoples R China

[2] Fudan Univ, Inst Med Microbiol, Shanghai 200032, Peoples R China

[3] Shanghai Municipal Ctr Dis Control & Prevent, Dept TB Control, Shanghai 200336, Peoples R China

[4] Ustar Biotechnol Hangzhou Co Ltd, Hangzhou 310012, Zhejiang, Peoples R China

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 03期

基金：

美国国家卫生研究院;

关键词：

MEDIATED ISOTHERMAL AMPLIFICATION; MICROSCOPY; PCR;

D O I：

10.1128/JCM.01528-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Cross-priming amplification (CPA) technology for tuberculosis diagnosis from sputum specimens was evaluated. The sensitivity of CPA from smear-and liquid culture-positive specimens was 96.9%, and that from smear-negative and liquid culture-positive specimens was 87.5%. The specificity of CPA in culture-negative specimens was 98.8%.

引用

页码：845 / 847

页数：3

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