Mistletoe lectin dissociates into catalytic and binding subunits before translocation across the membrane to the cytoplasm

被引：36

作者：

Agapov, II

Tonevitsky, AG

Moysenovich, MM

Maluchenko, NV

Weyhenmeyer, R

Kirpichnikov, MP

机构：

[1] State Res Ctr GNIIgenet, Moscow 113545, Russia

[2] Inst Transplantol & Artificial Organs, Moscow 123182, Russia

[3] Moscow MV Lomonosov State Univ, Fac Biol, Moscow 119899, Russia

[4] MADAUS AG, D-51109 Cologne, Germany

[5] Russian Acad Sci, Shemyakin & Ovchinnikov Inst Bioorgan Chem, Moscow 117871, Russia

来源：

FEBS LETTERS | 1999年 / 452卷 / 03期

基金：

俄罗斯基础研究基金会;

关键词：

mistletoe lectin; ribosome-inactivating protein; monoclonal antibody; membrane translocation;

D O I：

10.1016/S0014-5793(99)00639-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hybridomas producing monoclonal antibodies (mAbs) against the mistletoe lectin A-chain (MLA) were obtained to investigate the intracellular routing and translocation of ribosome-inactivating proteins. Anti-MLA mAb MNAS did not bind the holotoxin but interacted with isolated MLA. This epitope,vas not recognized upon MLA denaturation or conjugation of MLA with the ricin binding subunit (RTB), Furthermore, the mAbs did not appreciably react with a panel of MLA synthetic octapeptides linked to the surface of polyethylene pins. A study of the cytotoxicity of mistletoe lectin, ricin, and chimeric toxin MLA/RTB for the hybridomas revealed that interchain disulfide bond reduction and subunit dissociation are required for cytotoxic activity of mistletoe lectin, (C) 1999 Federation of European Biochemical Societies.

引用

页码：211 / 214

页数：4

共 21 条

[1] The role of structural domains in RIP II toxin model membrane binding [J].