Subcellular reorganization of mitochondria producing heavy DNA in aging wheat coleoptiles

被引：38

作者：

Bakeeva, LE ^{[1
]}

Kirnos, MD ^{[1
]}

Aleksandrushkina, NI ^{[1
]}

Kazimirchyuk, SB ^{[1
]}

Shorning, BY ^{[1
]}

Zamyatnina, VA ^{[1
]}

Yaguzhinsky, LS ^{[1
]}

Vanyushin, BF ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Belozersky Inst Physicochem Biol, Moscow 119899, Russia

来源：

FEBS LETTERS | 1999年 / 457卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

aging; apoptosis; DNA synthesis; heavy DNA; mitochondria; mitochondrial DNA; plant; wheat;

D O I：

10.1016/S0014-5793(99)01025-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Unusual closed membrane vesicles containing one or more mitochondria were isolated from homogenates of aging wheat coleoptiles, Very similar (or the same) bodies mere shown to exist in situ in vacuoles of undividing cells in the apical part of intact senescent coleoptiles, Vesicles isolated from coleoptile homogenate free of nuclei by 10 min centrifugation at 1700 X g and traditional mitochondria (sedimented at between 4300 X g and 17 400 X g) are similar in respiration rate, composition and content of cytochromes and sensitivity to respiration inhibitors. However, vesicles contain about 2-fold more Ca2+ ions than free mitochondria do. The specific feature of vesicles containing mitochondria in aging coleoptiles is an intensive synthesis of heavy (rho=1.718 g/cm(3)) mitochondrial DNA (H-mtDNA). Thus, aging in plants is accompanied by an increased selective H-mtDNA production and change in subcellular organization of mitochondria, (C) 1999 Federation of European Biochemical Societies.

引用

页码：122 / 125

页数：4

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