Cilostazol enhances IL-1β-induced NO production and apoptosis in rat vascular smooth muscle via PKA-dependent pathway

被引：18

作者：

Ito, C ^{[1
]}

Kusano, E ^{[1
]}

Akimoto, T ^{[1
]}

Takeda, S ^{[1
]}

Sasaki, N ^{[1
]}

Umino, T ^{[1
]}

Iimura, O ^{[1
]}

Ando, Y ^{[1
]}

Asano, Y ^{[1
]}

机构：

[1] Jichi Med Sch, Dept Nephrol, Minami Kawachi, Tochigi 3290498, Japan

来源：

CELLULAR SIGNALLING | 2002年 / 14卷 / 07期

关键词：

cilostazol; inducible nitric oxide synthase; vascular smooth muscle; interleukin-1; beta; apoptosis; protein kinase A;

D O I：

10.1016/S0898-6568(02)00004-9

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Interleukin-1beta (IL-1beta) stimulates nitric oxide (NO) production and induces apoptosis in several tissues. Cilostazol is a Type 3 phosphodiesterase inhibitor. We investigated whether cilostazol affects IL-1beta-induced NO production and apoptosis in rat vascular smooth muscle cells. Cilostazol (100 nM-10 muM) potentiated NO production triggered by IL-1beta. The mRNA and protein expression of inducible NO synthase was also upregulated by cilostazol. KT5720, an inhibitor of protein kinase A, and N-G-monomethyl-L-arginine, an inhibitor of NO synthase, abrogated cilostazol-enhanced IL-1beta-stimulated NO production and apoptosis. These results shows that cilostazol potentiates IL-1beta-induced NO production via PKA-pathway and thereafter augments apoptosis via NO-dependent pathway. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：625 / 632

页数：8

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