Cobalt but not hypoxia stimulates PDGF gene expression in rats

被引：8

作者：

Bucher, M ^{[1
]}

Sandner, P ^{[1
]}

Wolf, K ^{[1
]}

Kurtz, A ^{[1
]}

机构：

[1] UNIV REGENSBURG, INST PHYSIOL, D-93040 REGENSBURG, GERMANY

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM | 1996年 / 271卷 / 03期

关键词：

growth factors; oxygen sensing; divalent metals; anemia; carbon monoxide; platelet-derived growth factor;

D O I：

10.1152/ajpendo.1996.271.3.E451

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Cobalt but not hypoxia stimulates PDGF gene expression in rats. Am. J. Physiol. 271 (Endocrinol. Metab. 34): E451-E457, 1996.-This study was done to investigate the influence of different forms of acute tissue hypoxia on the expression of platelet-derived growth factor (PDGF) A chain (PDGF-A) and PDGF B chain (PDGF-B) genes in different rat organs. We found that acute normobaric hypoxia (8% O-2), carbon monoxide inhalation (0.1% CO), or lowering the hematocrit to 12% for 6 h had no effect on PDGF-A or PDGF-B gene expression in lung, heart, kidney, and liver of Sprague-Dawley rats. Subcutaneous administration of cobaltous chloride dose dependently increased PDGF-B mRNA by 125% in lungs, by 60% in kidneys, but not in heart and liver. These findings suggest that acute tissue; hypoxygenation is not a significant stimulus for PDGF-A and PDGF-B gene expression in these major rat organs. Cobalt appears to cause a tissue-specific increase of PDGF-B gene expression.

引用

页码：E451 / E457

页数：7

共 29 条

[1] MALIGNANT EPITHELIAL-CELLS IN PRIMARY HUMAN LUNG CARCINOMAS COEXPRESS INVIVO PLATELET-DERIVED GROWTH-FACTOR (PDGF) AND PDGF RECEPTOR MESSENGER-RNAS AND THEIR PROTEIN PRODUCTS [J].

ANTONIADES, HN ;

GALANOPOULOS, T ;

NEVILLEGOLDEN, J ;

OHARA, CJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (09) :3942-3946

[2] PLATELET-DERIVED GROWTH-FACTOR IN IDIOPATHIC PULMONARY FIBROSIS [J].

ANTONIADES, HN ;

BRAVO, MA ;

AVILA, RE ;

GALANOPOULOS, T ;

NEVILLEGOLDEN, J ;

MAXWELL, M ;

SELMAN, M .

JOURNAL OF CLINICAL INVESTIGATION, 1990, 86 (04) :1055-1064

[3] DIFFERENTIAL PROLIFERATION OF RAT LUNG FIBROBLASTS INDUCED BY THE PLATELET-DERIVED GROWTH FACTOR-AA, FACTOR-AB, AND FACTOR-BB ISOFORMS SECRETED BY RAT ALVEOLAR MACROPHAGES [J].

BONNER, JC ;

OSORNIOVARGAS, AR ;

BADGETT, A ;

BRODY, AR .

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1991, 5 (06) :539-547

[4] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[5]

COLLINS T, 1987, AM J PATHOL, V126, P7

[6] MECHANISMS OF PROGRESSIVE RENAL DISEASE IN GLOMERULONEPHRITIS [J].

COUSER, WG ;

JOHNSON, RJ .

AMERICAN JOURNAL OF KIDNEY DISEASES, 1994, 23 (02) :193-198

[7] OXYGEN-DEPENDENT EXPRESSION OF THE ERYTHROPOIETIN GENE IN RAT HEPATOCYTES INVITRO [J].

ECKARDT, KU ;

PUGH, CW ;

RATCLIFFE, PJ ;

KURTZ, A .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1993, 423 (5-6) :356-364

[8] INFUSION OF PLATELET-DERIVED GROWTH-FACTOR OR BASIC FIBROBLAST GROWTH-FACTOR INDUCES SELECTIVE GLOMERULAR MESANGIAL CELL-PROLIFERATION AND MATRIX ACCUMULATION IN RATS [J].

FLOEGE, J ;

ENG, E ;

YOUNG, BA ;

ALPERS, CE ;

BARRETT, TB ;

BOWENPOPE, DF ;

JOHNSON, RJ .

JOURNAL OF CLINICAL INVESTIGATION, 1993, 92 (06) :2952-2962

[9]

FLOEGE J, 1993, AM J PATHOL, V142, P637

[10] REGULATION OF ANGIOGENIC GROWTH-FACTOR EXPRESSION BY HYPOXIA, TRANSITION-METALS, AND CHELATING-AGENTS [J].

GLEADLE, JM ;

EBERT, BL ;

FIRTH, JD ;

RATCLIFFE, PJ .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1995, 268 (06) :C1362-C1368

← 1 2 3 →