Indicator cell lines for detection of primary strains of human and simian immunodeficiency viruses

被引:176
作者
Vodicka, MA
Goh, WC
Wu, LI
Rogel, ME
Bartz, SR
Schweickart, L
Raport, CJ
Emerman, M
机构
[1] FRED HUTCHINSON CANC RES CTR,DIV MOL MED,SEATTLE,WA 98109
[2] ICOS CORP,BOTHELL,WA 98021
关键词
D O I
10.1006/viro.1997.8606
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
CCR5 and CXCR4 are the two major coreceptors that have been identified for human immunodeficiency virus (HIV) entry. We have modified several beta-galactosidase-based HIV indicator cell lines to express CCR5 and/or CXCR4. Using these new reagents, we have been able to detect all primary isolates tested using one or both of these cell lines. However, there is large variation in the absolute viral infectivity among primary strains. Furthermore, all HIV strains are capable of causing syncytia in the indicator cells when the coreceptor is present regardless of whether they had previously been characterized as ''syncytia-inducing'' or ''non-syncytium-inducing.'' (C) 1997 Academic Press.
引用
收藏
页码:193 / 198
页数:6
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