Metabolism of pectin in the gastrointestinal tract

被引:13
作者
Dongowski, G
Anger, H
机构
[1] German Institute of Human Nutrition Potsdam-Rehbrücke, Department of Food Chemistry and Preventive Nutrition
来源
PECTINS AND PECTINASES | 1996年 / 14卷
关键词
D O I
10.1016/S0921-0423(96)80300-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Pectin was depolymerized and de-esterified only to a small extent after treatment under conditions simulating the gastrointestinal lumen. The degree of esterification was slightly decreased in the upper part of gastrointestinal tract of germfree and conventional rats and additionally in caecum and colon of germfree rats. Pectin passes the small intestine as a macromolecule. The molecular weight distribution of pectins measured by gel chromatography with viscosity detection fi om faeces of germfree rats remained nearly unchanged. No or very low amounts of galacturonan were found in contents of caecum, colon and in faeces of most of the conventional rats. Oligogalacturonic acids were not detected in faeces of these animals. Di- and trigalacturonic acid which could then be absorbed by the host were present in the colon of a few conventional rats. However during in vitro fermentation of pectin with human faecal flora unsaturated oligogalacturonic acids were found as intermediate products in variable concentration and composition within ca. 8 hours using thin-layer chromatography and HPLC (PAD, chiraliser and UV detection). Low-esterified pectin was fermented faster than high-esterified pectins.
引用
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页码:659 / 666
页数:8
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