Relationship between expression of p21WAF1/CIP1 radioresistance in human gliomas

被引：28

作者：

Kokunai, T ^{[1
]}

Tamaki, N ^{[1
]}

机构：

[1] Kobe Univ, Sch Med, Dept Neurosurg, Chuo Ku, Kobe, Hyogo 6500017, Japan

来源：

JAPANESE JOURNAL OF CANCER RESEARCH | 1999年 / 90卷 / 06期

关键词：

p21; radiosensitivity; glioma; apoptosis; p53;

D O I：

10.1111/j.1349-7006.1999.tb00795.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The role of p21(WAF1/CIPI) (p21) in DNA repair and apoptosis following gamma-irradiation remains controversial. In this study the influence of p21 on the radiosensitivity of human brain tumors was investigated. Resected tumors were stained immunohistochemically for p21, Expression of p21 in astrocytic tumors was high, but it was low in medulloblastomas, germinomas, and primary malignant lymphomas. Glioma and medulloblastoma cell lines were transfected with pcDNA/p21 to cause p21 overexpression, then tumor-cell colony formation and apoptosis were assessed following gamma-irradiation of the transfected and nontransfected cells. Overexpression of p21 enhanced clonogenic survival and suppressed apoptosis after gamma-irradiation in human brain turner cell lines with or without p53 protein deficiency Radioresistance was acquired when p21 was overproduced in the glioma cell lines irrespective of p53 status.

引用

页码：638 / 646

页数：9

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