An immunohistochemical analysis of heat shock protein 70, p53, and estrogen receptor status in carcinoma of the uterine cervix

被引:42
作者
Park, CS
Joo, IS
Song, SY
Kim, DS
Bae, DS
Lee, JH
机构
[1] Sungkyunkwan Univ, Dept Obstet & Gynecol, Kangnam Ku, Seoul 135710, South Korea
[2] Sungkyunkwan Univ, Dept Diagnost Pathol, Samsung Med Ctr, Sch Med,Kangnam Ku, Seoul 135710, South Korea
关键词
HSP; p53; ER; HPV; immunohistochemistry; cervical cancer;
D O I
10.1006/gyno.1999.5429
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Objectives. It has been shown that heat shock proteins (HSPs) protect cells from death caused by various noxious stimuli. Overexpression of HSP70 seems to be related to hormonal regulation of cell proliferation and/or down-regulation of sex steroid receptors, Wild-type p53 has been reported to repress HSP70 gene expression, It has been shown that mutant p53-HSP70 complex is highly expressed in cancer. However, the relationship between HSPs and steroid receptors or tumor suppressor gene products has not been well understood in uterine cervical carcinoma. This study was undertaken to examine the expression of HSP70, estrogen receptor (ER), and p53 in carcinoma of the uterine cervix, In addition, we analyzed HPV infection status and compared it to such immunohistochemical parameters. We also analyzed the relationship between these biological products and their clinicopathologic characteristics, Methods. Paraffin-embedded tissue sections were obtained from 84 patients with carcinoma of the uterine cervix. Expression of HSP70, p53, and ER was evaluated by immunohistochemical staining using anti-HSP70 monoclonal antibody (SPA810), anti-p53 (BP53.12), and ER1D5 antibody, respectively. PCR HPV detection was done by dot hybridization method. Results. Positive staining of HSP70 was detected in 73% of the cases. HSP70 positivity was significantly higher in stage I cervical cancer than in stages II-TV (P = 0.02). This was associated with neither tumor size, lymph node status, parametrial involvement status, nor tumor markers (TA-4). Furthermore, there was no significant correlation between HSP70 positivity and the expression of p53 or ER or HPV infection status. Conclusion. These data suggested that HSP70 positivity was frequent in uterine cervical cancer, especially in the early stages, However, this was not significantly correlated with clinicopathologic characteristics nor with the expression of p53 or ER nor with HPV infection in carcinoma of the uterine cervix. (C) 1999 Academic Press.
引用
收藏
页码:53 / 60
页数:8
相关论文
共 39 条
  • [1] REGULATION OF THE HUMAN HSP70 PROMOTER BY P53
    AGOFF, SN
    HOU, J
    LINZER, DIH
    WU, B
    [J]. SCIENCE, 1993, 259 (5091) : 84 - 87
  • [2] PROGESTERONE ENHANCES TARGET GENE-TRANSCRIPTION BY RECEPTOR FREE OF HEAT-SHOCK PROTEINS HSP90, HSP56, AND HSP70
    BAGCHI, MK
    TSAI, SY
    TSAI, MJ
    OMALLEY, BW
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (10) : 4998 - 5004
  • [3] P53 ACCUMULATION IN OVARIAN CARCINOMAS AND ITS PROGNOSTIC IMPLICATIONS
    BOSARI, S
    VIALE, G
    RADAELLI, U
    BOSSI, P
    BONOLDI, E
    COGGI, G
    [J]. HUMAN PATHOLOGY, 1993, 24 (11) : 1175 - 1179
  • [4] HEAT-SHOCK PROTEIN-HSP70 IN PATIENTS WITH AXILLARY LYMPH NODE-NEGATIVE BREAST-CANCER - PROGNOSTIC IMPLICATIONS
    CIOCCA, DR
    CLARK, GM
    TANDON, AK
    FUQUA, SAW
    WELCH, WJ
    MCGUIRE, WL
    [J]. JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1993, 85 (07) : 570 - 574
  • [5] BIOLOGICAL AND CLINICAL IMPLICATIONS OF HEAT-SHOCK PROTEIN 27000 (HSP27) - A REVIEW
    CIOCCA, DR
    OESTERREICH, S
    CHAMNESS, GC
    MCGUIRE, WL
    FUQUA, SAW
    [J]. JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1993, 85 (19): : 1558 - 1570
  • [6] CIOCCA DR, 1993, CANCER RES, V53, P4443
  • [7] IMMUNE-RESPONSE TO P53 IS DEPENDENT UPON P53/HSP70 COMPLEXES IN BREAST CANCERS
    DAVIDOFF, AM
    IGLEHART, JD
    MARKS, JR
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (08) : 3439 - 3442
  • [8] DIPAOLO JA, 1989, ONCOGENE, V4, P395
  • [9] HEAT-SHOCK ALTERS THE COMPOSITION OF HETEROMERIC STEROID-RECEPTOR COMPLEXES AND ENHANCES RECEPTOR ACTIVITY INVIVO
    EDWARDS, DP
    ESTES, PA
    FADOK, VA
    BONA, BJ
    ONATE, S
    NORDEEN, SK
    WELCH, WJ
    [J]. BIOCHEMISTRY, 1992, 31 (09) : 2482 - 2491
  • [10] ELLEDGE RM, 1994, CANCER RES, V54, P3752