Mass spectrometric analysis of neuropeptidergic systems in the human pituitary and cerebrospinal fluid

被引:26
作者
Desiderio, DM
机构
[1] Univ Tennessee, Dept Neurol, Charles B Stout Neurosci Mass Spectrometry Lab, Memphis, TN 38163 USA
[2] Univ Tennessee, Dept Biochem, Charles B Stout Neurosci Mass Spectrometry Lab, Memphis, TN 38163 USA
来源
JOURNAL OF CHROMATOGRAPHY B | 1999年 / 731卷 / 01期
关键词
reviews; neuropeptides; methionine enkephalin; beta-endorphin; proenkephalin A; proopiomelanocortin; tachykinin;
D O I
10.1016/S0378-4347(99)00172-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Neuropeptidergic systems have been studied in human tissues and fluids, which include the pituitary and lumbar cerebrospinal fluid, respectively. This paper reviews the qualitative and quantitative mass spectrometric analytical data obtained from three areas of study. Methionine enkephalin (ME) and beta-endorphin (BE) were quantified in the human pituitary by liquid secondary ion mass spectrometry (LSI MS)-tandem mass spectrometry. Corresponding stable isotope incorporated synthetic peptide internal standards were used. Proenkephalin A and proopiomelanocortin produce ME and BE, respectively. The analysis of neuropeptides in macroadenomas demonstrated a decrease in both of those neuropeptidergic systems relative to controls. An analysis of prolactin-secreting microadenomas showed an increase in the proenkephalin A system. Mass spectrometry was also used to detect opioid peptide-containing proteins in the pituitary. Enzymes that process the precursors of proenkephalin A and tachykinin (substance P) neuropeptides were studied in human lumbar cerebrospinal fluid. Electrospray ionization mass spectrometry was used to characterize the molecular mass of each peptide product. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:3 / 22
页数:20
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