Comparison of partial 16S rRNA gene sequences obtained from activated sludge bacteria

被引:25
作者
Vainio, EJ
Moilanen, A
Koivula, TT
Bamford, DH
Hantula, J
机构
[1] FOREST RES INST,FIN-01301 VANTAA,FINLAND
[2] UNIV HELSINKI,DEPT ECOL & SYSTEMAT,DIV POPULAT BIOL,FIN-00014 HELSINKI,FINLAND
[3] UNIV HELSINKI,DEPT BIOSCI,DIV MICROBIOL,FIN-00014 HELSINKI,FINLAND
[4] UNIV HELSINKI,DEPT BIOSCI,DIV GENET,FIN-00014 HELSINKI,FINLAND
[5] UNIV HELSINKI,INST BIOTECHNOL,FIN-00014 HELSINKI,FINLAND
关键词
D O I
10.1007/s002530051017
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The cultivated and uncultivated bacterial communities of an activated sludge plant were studied. Two samples were taken and a total of 516 bacterial isolates were classified into groups using their whole-cell protein patterns. The distribution of bacteria into protein-pattern groups differed significantly between tl-le two samples, suggesting variation in culturable bacterial flora. Partial 16S rRNA gene sequences were determined for representatives of the commonest protein-pattern groups. Most of the sequences obtained were previously unknown, but relatively closely related to known sequences of organisms belonging to the alpha, beta or gamma subclasses of the proteobacteria, the first two subclasses being predominant. This classification of bacteria isolated on a diluted nutrient-rich medium differed from recent culture-dependent studies using nutrient-rich media. The uncultivated bacterial community was studied by analyzing ten partial 16S rRNA gene sequences cloned directly from activated sludge. None of the cloned sequences was identical to those determined for culturable organisms; or to those in the GenBank database. They were, however, related to the alpha or beta subclasses of the proteobacteria, or to the gram-positive bacteria with a high G + C DNA content.
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页码:73 / 79
页数:7
相关论文
共 36 条
[1]   The bacteriology of activated sludge [J].
Allen, LA .
JOURNAL OF HYGIENE, 1944, 43 (06) :424-431
[2]   IDENTIFICATION INSITU AND PHYLOGENY OF UNCULTURED BACTERIAL ENDOSYMBIONTS [J].
AMANN, R ;
SPRINGER, N ;
LUDWIG, W ;
GORTZ, HD ;
SCHLEIFER, KH .
NATURE, 1991, 351 (6322) :161-164
[3]   PHYLOGENETIC IDENTIFICATION AND IN-SITU DETECTION OF INDIVIDUAL MICROBIAL-CELLS WITHOUT CULTIVATION [J].
AMANN, RI ;
LUDWIG, W ;
SCHLEIFER, KH .
MICROBIOLOGICAL REVIEWS, 1995, 59 (01) :143-169
[4]  
American Public Health Association, 1985, STAND METH EX WAT WA
[5]  
BAIROCH A, 1992, PC GENE NUCL ACID PR
[6]   AEROBIC HETEROTROPHIC BACTERIA IN ACTIVTED SLUDGE [J].
BENEDICT, RG ;
CARLSON, DA .
WATER RESEARCH, 1971, 5 (11) :1023-&
[7]  
BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513
[8]  
BLAIM H, 1984, Z WASSER ABWASS FOR, V17, P37
[9]   BACTERIAL COMMUNITY STRUCTURES OF PHOSPHATE-REMOVING AND NON-PHOSPHATE-REMOVING ACTIVATED SLUDGES FROM SEQUENCING BATCH REACTORS [J].
BOND, PL ;
HUGENHOLTZ, P ;
KELLER, J ;
BLACKALL, LL .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (05) :1910-1916
[10]   GENBANK [J].
BURKS, C ;
CINKOSKY, MJ ;
FISCHER, WM ;
GILNA, P ;
HAYDEN, JED ;
KEEN, GM ;
KELLY, M ;
KRISTOFFERSON, D ;
LAWRENCE, J .
NUCLEIC ACIDS RESEARCH, 1992, 20 :2065-2069