Microbial symbionts of the Australian Great Barrier Reef sponge, Candidaspongia flabellata

被引:31
作者
Burja, AM
Hill, RT
机构
[1] Univ Maryland, Ctr Marine Biotechnol, Inst Biotechnol, Baltimore, MD 21202 USA
[2] Australian Inst Marine Sci, Marine Biodivers Med Ind & Environm Grp, Townsville, Qld 4810, Australia
关键词
16S rRNA; marine bacteria; Dictyoceratida; alpha-proteobacteria; cyanobacteria;
D O I
10.1023/A:1012713130404
中图分类号
Q17 [水生生物学];
学科分类号
071004 ;
摘要
Microbial symbionts of the newly described rare, biochemically active Dictyoceratid sponge, Candidaspongia flabellata (Very White Fan) found in the Australian Great Barrier Reef, are being Studied in detail. The chemistry of this sponge species is distinctive, and includes a previously undescribed compound, fanolide as well as homosesterterpene and bishomoscalarane secondary metabolites (Bergquist et al., 1999). Current research is focused on assessing (lie diversity of the microbial community associated with this sponge. The entire culturable community of this sponge has been studied in detail. A total of 228 bacteria. 25 fungi, 3 actinomycetes and 9 cyanobacteria were isolated from 10 individuals of this sponge, Eight eubacteria (designated AB001-AB008), along with seven cyanobacteria were consistently found associated with C. flabellata and absent from the surrounding water column, suggesting that these bacteria have a specific association with the sponge. Partial 16S ribosomal RNA gene sequencing of these isolates was done for phylogenetic characterisation. Electron microscopy was also used to confirm the presence of many morphotypes of bacteria and indicated spatial arrangements of particular morphotypes.
引用
收藏
页码:41 / 47
页数:7
相关论文
共 29 条