Protective effects of probiotic Lactobacillus casei Zhang against endotoxin- and D-galactosamine-induced liver injury in rats via anti-oxidative and anti-inflammatory capacities

被引：106

作者：

Wang, Yuzhen ^{[1
]}

Li, Yunxu ^{[1
]}

Xie, Jiming ^{[2
]}

Zhang, Yong ^{[3
]}

Wang, Jinling ^{[4
]}

Sun, Xiaolin ^{[1
]}

Zhang, Heping ^{[3
]}

机构：

[1] Inner Mongolia Agr Univ, Coll Life Sci, Hohhot 010018, Peoples R China

[2] Inner Mongolia Peoples Hosp, Clin Lab, Hohhot 010010, Peoples R China

[3] Inner Mongolia Agr Univ, Coll Food Sci & Engn, Educ Minist China, Key Lab Dairy Biotechnol & Bioengn, Hohhot 010018, Peoples R China

[4] Inner Mongolia Agr Univ, Coll Vet Med, Hohhot 010018, Peoples R China

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2013年 / 15卷 / 01期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

Lactobacillus casei Zhang; Lipopolysaccharide; D-Galactosamine; Liver injury; THF-alpha; TLR4; NITRIC-OXIDE PRODUCTION; NECROSIS-FACTOR-ALPHA; KAPPA-B ACTIVATION; HEPATIC-INJURY; MOUSE MODEL; MICE; LIPOPOLYSACCHARIDE; EXPRESSION; BACTERIA; DEGRADATION;

D O I：

10.1016/j.intimp.2012.10.026

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Lactobacillus casei Zhang (LcZ) has been recently isolated from the traditional Mongolian beverage koumiss and has a set of favorable probiotic properties, including aciduricity, bile resistance and ability to colonize the gastrointestinal tract. We have previously reported the anti-oxidative properties of LcZ in the hyperlipidemic rats. In this study, the hepatoprotective effects of LcZ against lipopolysaccharide (LPS) and D-galactosamine (D-GalN)-induced liver injury were investigated. We found that pretreatment with LcZ significantly improved survival of rats challenged with LPS/D-GalN. In addition, pretreatment with LcZ significantly decreased alanine transaminase (ALT) and aspartate aminotransferase (AST) levels in LPS/D-GalN-challenged rats, which were accompanied by diminished liver injuries, reduced malondialdehyde (MDA) content and increased superoxide dismutase (SOD) activity in liver homogenates. Pretreatment with LcZ also markedly reduced LPS/D-GalN-induced production of hepatic nitric oxide (NO), activation of inducible nitric oxide synthase (iNOS) and expression of tumor necrosis factor-alpha (TNF-alpha). Furthermore, hepatic toll-like receptor 4 (TLR4) mRNA and protein levels, the phosphorylation of I-kappa B and translocation of nuclear factor kappa B (NF-kappa B) were significantly down-regulated by pretreatment with LcZ. These results suggest that pretreatment with LcZ protects against LPS/D-GalN-induced liver injury in rats via its anti-oxidative and anti-inflammatory capacities. The hepatoprotective effects of LcZ are associated with an inhibition of TLR4 expression and TLR4 signaling. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：30 / 37

页数：8

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