Evaluation of the chemiluminescent nitrogen detector for solubility determinations to support drug discovery

被引：40

作者：

Bhattachar, SN ^{[1
]}

Wesley, JA ^{[1
]}

Seadeek, C ^{[1
]}

机构：

[1] Pfizer Global Res & Dev, Ann Arbor Labs, Ann Arbor, MI 48105 USA

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2006年 / 41卷 / 01期

关键词：

chemiluminescent nitrogen detection; solubility; drug discovery;

D O I：

10.1016/j.jpba.2005.11.009

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Solubility measurements using chemiluminescent nitrogen detection (CLND) has advantages of reduced compound requirement and increased throughput compared to UV-spectrophotometric and HPLC-based measurements. CLND with direct flow injection was evaluated for the measurement of thermodynamic solubility to support drug discovery. The limit of quantitation (LOQ), accuracy, and day-to-day reproducibility of the detector were measured. Measurements made on CLND were compared to those obtained from UV spectrophotometry and HPLC. Based on the results obtained, it was concluded that the CLND performs satisfactorily for discovery stage thermodynamic solubility measurements. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：152 / 157

页数：6

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