Organization and expression analysis of the zebrafish hepcidin gene, an antimicrobial peptide gene conserved among vertebrates

被引：128

作者：

Shike, H ^{[1
]}

Shimizu, C ^{[1
]}

Lauth, X ^{[1
]}

Burns, JC ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Pediat, La Jolla, CA 92093 USA

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2004年 / 28卷 / 7-8期

关键词：

fish; Streptococcus iniae; infection; hepcidin; bacterial challenge;

D O I：

10.1016/j.dci.2003.11.009

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Hepcidin is an antimicrobial peptide and iron-regulatory molecule that is conserved among vertebrates. Mutations or overexpression of the human hepcidin gene have been found in patients with hemochromatosis and refractory anemia. To further understand the function and regulation of hepcidin, animal models are needed. We sequenced cDNA, genes and upstream regions of zebrafish hepcidin and analyzed gene expression by kinetic PCR. Zebrafish hepcidin genes consist of two introns and three exons that encode a prepropeptide (91 amino acids). The amino acid sequences and gene organization were remarkably conserved between zebrafish and other species. Elevated gene expression was observed in abdominal organs, skin, and heart in fish that developed signs of infection following bacterial injection. Zebrafish may be a suitable model organism for further study of hepcidin gene regulation. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：747 / 754

页数：8

共 23 条

[1] Toll signaling pathways in the innate immune response
Anderson, KV
[J]. CURRENT OPINION IN IMMUNOLOGY, 2000, 12 (01) : 13 - 19
[2] C/EBPα regulates hepatic transcription of hepcidin, an antimicrobial peptide and regulator of iron metabolism
Courselaud, B
Pigeon, C
Inoue, Y
Inoue, J
Gonzalez, FJ
Leroyer, P
Gilot, D
Boudjema, K
Guguen-Guillouzo, C
Brissott, P
Loréal, O
Ilyin, G
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (43) : 41163 - 41170
[3] Identification and expression analysis of hepcidin-like antimicrobial peptides in bony fish
Douglas, SE
Gallant, JW
Liebscher, RS
Dacanay, A
Tsoi, SCM
[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2003, 27 (6-7) : 589 - 601
[4] Streptococcus iniae virulence is associated with a distinct genetic profile
Fuller, JD
Bast, DJ
Nizet, V
Low, DE
de Azavedo, JCS
[J]. INFECTION AND IMMUNITY, 2001, 69 (04) : 1994 - 2000
[5] Hypoxia-induced gene expression profiling in the euryoxic fish Gillichthys mirabilis
Gracey, AY
Troll, JV
Somero, GN
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (04) : 1993 - 1998
[6] CORRELATION BETWEEN STABILITY OF A PROTEIN AND ITS DIPEPTIDE COMPOSITION - A NOVEL-APPROACH FOR PREDICTING INVIVO STABILITY OF A PROTEIN FROM ITS PRIMARY SEQUENCE
GURUPRASAD, K
REDDY, BVB
PANDIT, MW
[J]. PROTEIN ENGINEERING, 1990, 4 (02): : 155 - 161
[7] Databases on transcriptional regulation: TRANSFAC, TRRD and COMPEL
Heinemeyer, T
Wingender, E
Reuter, I
Hermjakob, H
Kel, AE
Kel, OV
Ignatieva, EV
Ananko, EA
Podkolodnaya, OA
Kolpakov, FA
Podkolodny, NL
Kolchanov, NA
[J]. NUCLEIC ACIDS RESEARCH, 1998, 26 (01) : 362 - 367
[8] Comparative analysis of mouse hepcidin 1 and 2 genes:: evidence for different patterns of expression and co-inducibility during iron overload
Ilyin, G
Courselaud, B
Troadec, MB
Pigeon, C
Alizadeh, M
Leroyer, P
Brissot, P
Loréal, O
[J]. FEBS LETTERS, 2003, 542 (1-3) : 22 - 26
[9] Inoue S, 1997, MOL MAR BIOL BIOTECH, V6, P376
[10] Kang J J, 2000, Nucleic Acids Res, V28, pe2, DOI 10.1093/nar/28.2.e2

← 1 2 3 →