Detection of Circulating Pancreas Epithelial Cells in Patients With Pancreatic Cystic Lesions

被引：211

作者：

Rhim, Andrew D. ^{[1
,2
,3
]}

Thege, Fredrik I. ^{[6
]}

Santana, Steven M. ^{[7
]}

Lannin, Timothy B. ^{[7
]}

Saha, Trisha N. ^{[1
,2
]}

Tsai, Shannon ^{[2
,3
]}

Maggs, Lara R. ^{[2
,3
]}

Kochman, Michael L. ^{[2
,3
,5
]}

Ginsberg, Gregory G. ^{[2
,3
,5
]}

Lieb, John G. ^{[2
,3
]}

Chandrasekhara, Vinay ^{[2
,3
]}

Drebin, Jeffrey A. ^{[3
,4
]}

Ahmad, Nuzhat ^{[2
]}

Yang, Yu-Xiao ^{[2
]}

Kirby, Brian J. ^{[7
,8
]}

Stanger, Ben Z. ^{[2
,3
,5
]}

机构：

[1] Univ Michigan, Sch Med, Dept Internal Med, Div Gastroenterol,Pancreat Canc Ctr,Comprehens Ca, Ann Arbor, MI 48108 USA

[2] Univ Penn, Perelman Sch Med, Dept Med, Div Gastroenterol, Philadelphia, PA 19104 USA

[3] Univ Penn, Perelman Sch Med, Abramson Canc Ctr, Philadelphia, PA 19104 USA

[4] Univ Penn, Perelman Sch Med, Dept Surg, Philadelphia, PA 19104 USA

[5] Univ Penn, Perelman Sch Med, Dept Cell & Dev Biol, Philadelphia, PA 19104 USA

[6] Cornell Univ, Dept Biomed Engn, Ithaca, NY USA

[7] Cornell Univ, Sibley Sch Mech & Aerosp Engn, Ithaca, NY 14853 USA

[8] Weill Cornell Med Coll, Dept Med, Div Hematol Med Oncol, New York, NY USA

来源：

GASTROENTEROLOGY | 2014年 / 146卷 / 03期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

Early Detection; IPMN; Circulating Tumor Cells; Pancreatic Cancer;

D O I：

10.1053/j.gastro.2013.12.007

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

100201 [内科学];

摘要：

Hematogenous dissemination is thought to be a late event in cancer progression. We recently showed in a genetic model of pancreatic ductal adenocarcinoma that pancreas cells can be detected in the bloodstream before tumor formation. To confirm these findings in humans, we used microfluidic geometrically enhanced differential immunocapture to detect circulating pancreas epithelial cells in patient blood samples. We captured more than 3 circulating pancreas epithelial cells/mL in 7 of 21 (33%) patients with cystic lesions and no clinical diagnosis of cancer (Sendai criteria negative), 8 of 11 (73%) with pancreatic ductal adenocarcinoma, and in 0 of 19 patients without cysts or cancer (controls). These findings indicate that cancer cells are present in the circulation of patients before tumors are detected, which might be used in risk assessment.

引用

页码：647 / 651

页数：5

共 9 条

[1]

Survival in pancreatic carcinoma based on tumor size [J].