Expression and roles of Cl- channel ClC-5 in cell cycles of myeloid cells

被引：25

作者：

Jiang, BH ^{[1
]}

Hattori, N ^{[1
]}

Liu, B ^{[1
]}

Nakayama, Y ^{[1
]}

Kitagawa, K ^{[1
]}

Sumita, K ^{[1
]}

Inagaki, C ^{[1
]}

机构：

[1] Kansai Med Univ, Dept Pharmacol, Moriguchi, Osaka 5708506, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 317卷 / 01期

关键词：

proliferation; cell cycle; ClC-5; myeloid cell;

D O I：

10.1016/j.bbrc.2004.03.036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

CIC-5 is a chloride channel known to be expressed in the kidney. We previously reported that CIC-5 mRNA was also strongly expressed in immature human myeloid cell line (HL-60), but weakly expressed in mature neutrophils. To clarify the underlying mechanisms, we examined the relationship between CIC-5 expression and cell cycle. Dimethyl sulfoxide treatment of HL-60 that causes differentiation with G0/G1 cell cycle arrest decreased the expression of CIC-5 rnRNA. Cell sorting and synchronization experiments revealed that CIC-5 rnRNA expression was high in S and G2/M phases and low in G0/G1 phase. CIC-5 antisense oligonucleotide suppressed proliferation of HL-60 cells with a decrease in CIC-5 protein expression, probably due to G2 arrest. These results suggest that cell cycle-dependent expression of CIC-5 has a role in cell cycle progression in myeloid cells. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：192 / 197

页数：6

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