Molecular cloning of a murine type III sodium-dependent phosphate cotransporter (Pit-2) gene promoter

被引:6
作者
Bai, LQ
Collins, JF
Xu, H
Xu, LP
Ghishan, FK
机构
[1] Univ Arizona, Hlth Sci Ctr, Steele Mem Childrens Res Ctr, Dept Pediat, Tucson, AZ 85724 USA
[2] Univ Arizona, Hlth Sci Ctr, Steele Mem Childrens Res Ctr, Dept Physiol, Tucson, AZ 85724 USA
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2001年 / 1522卷 / 01期
关键词
sodium-phosphate cotransporter; gene regulation; Caco-2; cell; OKP cell; intestine; Ram-1;
D O I
10.1016/S0167-4781(01)00297-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report the novel cloning and preliminary characterization of a murine type III sodium-dependent phosphate cotransporter (Pit-2) gene promoter. Five promoter/luciferase reporter gene constructs, -1816/+61, -1620/+61, -1223/+61, -6001+61 and -225/+61, showed significant luciferase activity (6-14-fold over background) when transfected into human colon carcinoma (Caco-2) and opossum kidney (OKP) cells. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:42 / 45
页数:4
相关论文
共 19 条
[1]  
[Anonymous], BIOCH BIOPHYS RES CO
[2]   Cloning and characterization of a type IIINa-dependent phosphate cotransporter from mouse intestine [J].
Bai, L ;
Collins, JF ;
Ghishan, FK .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2000, 279 (04) :C1135-C1143
[3]  
BAI L, 2001, AM J PHYSIOL, V288, pC1168
[4]   MATURATION OF JEJUNAL PHOSPHATE-TRANSPORT BY RAT BRUSH-BORDER MEMBRANE-VESICLES [J].
BOROWITZ, SM ;
GHISHAN, FK .
PEDIATRIC RESEARCH, 1985, 19 (12) :1308-1312
[5]   PHOSPHATE-TRANSPORT IN BRUSH-BORDER MEMBRANES FROM CONTROL AND RACHITIC PIG-KIDNEY AND SMALL-INTESTINE [J].
BRANDIS, M ;
HARMEYER, J ;
KAUNE, R ;
MOHRMANN, M ;
MURER, H ;
ZIMOLO, Z .
JOURNAL OF PHYSIOLOGY-LONDON, 1987, 384 :479-490
[6]  
CROSS HS, 1982, ADV EXP MED BIOL, V151, P127
[7]  
DENISI D, 1980, PFLUGERS ARCH, V388, P227
[8]  
DENISI D, 1990, SCAND J GASTROENTERO, V25, P210
[9]   Regulation of small intestinal Na-Pi type IIb cotransporter by dietary phosphate intake [J].
Hattenhauer, O ;
Traebert, M ;
Murer, H ;
Biber, J .
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 1999, 277 (04) :G756-G762
[10]   REGULATION OF NA+-PI COTRANSPORT BY 1,25-DIHYDROXYVITAMIN D3 IN RABBIT DUODENAL BRUSH-BORDER MEMBRANE [J].
HILDMANN, B ;
STORELLI, C ;
DANISI, G ;
MURER, H .
AMERICAN JOURNAL OF PHYSIOLOGY, 1982, 242 (05) :G533-G539