Molecular cloning of a murine type III sodium-dependent phosphate cotransporter (Pit-2) gene promoter

被引：6

作者：

Bai, LQ

Collins, JF

Xu, H

Xu, LP

Ghishan, FK

机构：

[1] Univ Arizona, Hlth Sci Ctr, Steele Mem Childrens Res Ctr, Dept Pediat, Tucson, AZ 85724 USA

[2] Univ Arizona, Hlth Sci Ctr, Steele Mem Childrens Res Ctr, Dept Physiol, Tucson, AZ 85724 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2001年 / 1522卷 / 01期

关键词：

sodium-phosphate cotransporter; gene regulation; Caco-2; cell; OKP cell; intestine; Ram-1;

D O I：

10.1016/S0167-4781(01)00297-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the novel cloning and preliminary characterization of a murine type III sodium-dependent phosphate cotransporter (Pit-2) gene promoter. Five promoter/luciferase reporter gene constructs, -1816/+61, -1620/+61, -1223/+61, -6001+61 and -225/+61, showed significant luciferase activity (6-14-fold over background) when transfected into human colon carcinoma (Caco-2) and opossum kidney (OKP) cells. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：42 / 45

页数：4

共 19 条

[1]

[Anonymous], BIOCH BIOPHYS RES CO

[2] Cloning and characterization of a type IIINa-dependent phosphate cotransporter from mouse intestine [J].

Bai, L ;

Collins, JF ;

Ghishan, FK .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2000, 279 (04) :C1135-C1143

[3]

BAI L, 2001, AM J PHYSIOL, V288, pC1168

[4] MATURATION OF JEJUNAL PHOSPHATE-TRANSPORT BY RAT BRUSH-BORDER MEMBRANE-VESICLES [J].

BOROWITZ, SM ;

GHISHAN, FK .

PEDIATRIC RESEARCH, 1985, 19 (12) :1308-1312

[5] PHOSPHATE-TRANSPORT IN BRUSH-BORDER MEMBRANES FROM CONTROL AND RACHITIC PIG-KIDNEY AND SMALL-INTESTINE [J].

BRANDIS, M ;

HARMEYER, J ;

KAUNE, R ;

MOHRMANN, M ;

MURER, H ;

ZIMOLO, Z .

JOURNAL OF PHYSIOLOGY-LONDON, 1987, 384 :479-490

[6]

CROSS HS, 1982, ADV EXP MED BIOL, V151, P127

[7]

DENISI D, 1980, PFLUGERS ARCH, V388, P227

[8]

DENISI D, 1990, SCAND J GASTROENTERO, V25, P210

[9] Regulation of small intestinal Na-Pi type IIb cotransporter by dietary phosphate intake [J].

Hattenhauer, O ;

Traebert, M ;

Murer, H ;

Biber, J .

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 1999, 277 (04) :G756-G762

[10] REGULATION OF NA+-PI COTRANSPORT BY 1,25-DIHYDROXYVITAMIN D3 IN RABBIT DUODENAL BRUSH-BORDER MEMBRANE [J].

HILDMANN, B ;

STORELLI, C ;

DANISI, G ;

MURER, H .

AMERICAN JOURNAL OF PHYSIOLOGY, 1982, 242 (05) :G533-G539

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