Induction of apoptosis of monocyte-macrophage lineage cells by 5-S-GAD

被引：8

作者：

Hijikata, M

Matsumoto, HN

Kobayashi, A

Nifuji, A

Noda, M

Natori, S

机构：

[1] RIKEN, Inst Phys & Chem Res, Natori Special Lab, Wako, Saitama 3510198, Japan

[2] Tokyo Med & Dent Univ, Inst Med Res, Dept Mol Pharmacol, Chiyoda Ku, Tokyo 113, Japan

[3] Tokyo Med & Dent Univ, Inst Biomat & Bioengn, Div Biosyst, Dept Biomed Engn, Tokyo 113, Japan

[4] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

来源：

FEBS LETTERS | 1999年 / 457卷 / 03期

基金：

日本学术振兴会;

关键词：

osteoclast; hydrogen peroxide; apoptosis; HL60; cell;

D O I：

10.1016/S0014-5793(99)01077-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We found that 5-S-GAD, an insect-derived antibacterial peptide, inhibited murine osteoclast formation in vitro. We examined the specific time point of the inhibitory action of 5-S-GAD on osteoclast formation and found that it mainly suppressed differentiation of osteoclasts in the middle of the culture period. Using HL60, cells that are able to differentiate into multinucleated macrophage-like cells, we found that 5-S-GAD induced apoptosis of HL60 cells by producing H2O2 Thus, the inhibition of osteoclast formation by 5-S-GAD could be, in part, due to apoptosis of the cells of an osteoclast lineage. (C) 1999 Federation of European Biochemical Societies.

引用

页码：405 / 408

页数：4

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